TAO Kinase 3 (TAOK3) (C-Term) antibody

Antigen: TAO Kinase 3 (TAOK3)

Synonyms: DPK, JIK, MAP3K18, FLJ31808, DKFZp666H245, A130052D22, 2900006A08Rik, A430105I05Rik, TAOK3, DKFZp459L243

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392392

Additional Information

Alternative name: JIK

Gene ID: 51347

UniProt: Q9UHG7

Immunogen: This JIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389~418 amino acids from the C-terminal region of human JIK.

Isotype: Ig

Description: Other names: STE20-like kinase, TAOK3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This JIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389~418 amino acids from the C-terminal region of human JIK.

Molecular Weight: 105657 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of JIK (arrow) using rabbit polyclonal JIK Antibody (C-term) (ABIN392392). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TAOK3 gene (Lane 2) (Origene Technologies).

Publications

Product: Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).