P21-Activated Kinase 1 (PAK1) (N-Term) antibody

Antigen: P21-Activated Kinase 1 (PAK1)

Synonyms: PAKalpha, MGC130000, MGC130001, Paka, PAK-1, AW045634, PAK1, MGC83445, MGC139676

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392431

Additional Information

Alternative name: PAK1

Gene ID: 5058

UniProt: Q13153

Immunogen: This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the N-terminal region of human PAK1.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, P65-PAK, Alpha-PAK

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140~169 amino acids from the N-terminal region of human PAK1.

Molecular Weight: 60661 DA

Comments:

Background: PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of PAK1(arrow) using rabbit polyclonal PAK1 antibody (N-term) (ABIN392431). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Chen, Yin, Zhu et al.: "The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20029-36, 2003 (PubMed).