PAK1 antibody (P21-Activated Kinase 1) (AA 401-430)

Details for Product anti-PAK1 Antibody No. ABIN392432, Supplier: Log in to see
Antigen
  • X-PAK1
  • XPak1
  • pak
  • pak-1
  • pakalpha
  • PAKalpha
  • AW045634
  • PAK-1
  • Paka
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • Protein PAK-1
  • pak1
  • PAK1
  • Pak1
  • pak-1
Alternatives
anti-Human PAK1 antibody for Dot Blot
Epitope
AA 401-430, Thr423
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Reactivity
Human
342
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Host
Rabbit
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Clonality (Clone)
Polyclonal ()
Conjugate
This PAK1 antibody is un-conjugated
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Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
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Immunogen This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 AA from human PAK1.
Clone RB11382
Isotype Ig
Specificity This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T423 of human PAK1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus),Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name PAK1 (PAK1 Antibody Abstract)
Background PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.
Synonyms: Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, P65-PAK, Alpha-PAK
Molecular Weight 60661 DA
Gene ID 5058
UniProt Q13153
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis
Application Notes WB = 1:1000, IF = 1:10-50, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.43 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-PAK1 antibody (P21-Activated Kinase 1) (AA 401-430) (ABIN392432) Western blot analysis of PAK1 (arrow) using rabbit polyclonal PAK1 Antibody (T423) (A...
Immunohistochemistry (IHC) image for anti-PAK1 antibody (P21-Activated Kinase 1) (AA 401-430) (ABIN392432) Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 A...
Background publications Chen, Yin, Zhu, Yan, Ji, Chen, Cai, Zhang, Zong, Hu, Yuan, Shen, Gu: "The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20029-36, 2003 (PubMed).