Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 6 (RPS6KA6) (N-Term) antibody

Antigen: Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 6 (RPS6KA6)

Synonyms: RSK4, PP90RSK4

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Primate

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392458

Additional Information

Alternative name: RSK4

Gene ID: 27330

UniProt: Q9UK32

Immunogen: This RSK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23~52 amino acids from the N-terminal region of human RSK4.

Isotype: Ig

Description: Other names: Ribosomal protein S6 kinase alpha 6, RPS6KA6

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RSK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23~52 amino acids from the N-terminal region of human RSK4.

Molecular Weight: 83871 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Neurology, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

RSK4 Antibody (N-term) (ABIN392458) is used to detect RSK4 in primate brain tissue lysate (lane 2).

RSK4 Antibody (N-term) (ABIN392458) is used to detect RSK4 in 7 different cell lines. Data courtesy of Dr. Yuan Sun, Hormel Institute, University of Minnesota.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Yntema, van den Helm, Kissing et al.: "A novel ribosomal S6-kinase (RSK4; RPS6KA6) is commonly deleted in patients with complex X-linked mental retardation." in: Genomics, Vol. 62, Issue 3, pp. 332-43, 2000 (PubMed).