Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Ser222) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2)

Synonyms: MEK2, MKK2, MAPKK2, PRKMK2, FLJ26075

Binding Site: Ser222

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392487

Additional Information

Alternative name: MAP2K2

Gene ID: 5605

UniProt: P36507

Immunogen: This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S222 of human MAP2K2.

Isotype: Ig

Description: Other names: Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S222 of human MAP2K2.

Molecular Weight: 44424 DA

Comments:

Background: MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blot~~1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of MAP2K2-pS222 (ABIN392487) in Y79 cell line lysates (35ug/lane). MAP2K2 (arrow) was detected using the purified Pab.

Western blot analysis of MAP2K2 (arrow) using rabbit polyclonal MAP2K2-pS222.ctrl (ABIN392487). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) .

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) (ABIN392487), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Liu, Jana, Dasgupta et al.: "Human immunodeficiency virus type 1 (HIV-1) tat induces nitric-oxide synthase in human astroglia." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39312-9, 2002 (PubMed).

Li, Inoki, Vacratsis et al.: "The p38 and MK2 kinase cascade phosphorylates tuberin, the tuberous sclerosis 2 gene product, and enhances its interaction with 14-3-3." in: The Journal of biological chemistry, Vol. 278, Issue 16, pp. 13663-71, 2003 (PubMed).

Burroughs, Oh, Barrett et al.: "Phosphatidylinositol 3-kinase and mek1/2 are necessary for insulin-like growth factor-I-induced vascular endothelial growth factor synthesis in prostate epithelial cells: a role for hypoxia-inducible factor-1?" in: Molecular cancer research : MCR, Vol. 1, Issue 4, pp. 312-22, 2003 (PubMed).