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Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (Tyr181) antibody

Antigen

Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)
Synonyms IKBA, MAD-3, NFKBI
Binding Site
Alternatives

Tyr181
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN392517
Quantity 0.1 mg  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (Tyr181) antibody

anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (Tyr181) antibody (2)

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name NFKBIA
Gene ID 4792
UniProt P25963
Immunogen This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164~193 amino acids surrounding Y181 of human NFKBIA.
Isotype Ig
Description Other names: NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Characteristics Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164~193 amino acids surrounding Y181 of human NFKBIA.
Molecular Weight 35478 DA
Comments

Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).

Application Details
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Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Western blot~~1:50~100 Immunohistochemistry ~~ 1:10~50
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Apoptosis/Necrosis, Cell Signaling, Transcription Factors, Cell Structure
Restrictions For Research Use only

Publications
Application Details Images Alternatives back to top

Product Gil, Alcamí, Esteban: "Activation of NF-kappa B by the dsRNA-dependent protein kinase, PKR involves the I kappa B kinase complex." in: Oncogene, Vol. 19, Issue 11, pp. 1369-78, 2000 (PubMed).

Tojima, Fujimoto, Delhase et al.: "NAK is an IkappaB kinase-activating kinase." in: Nature, Vol. 404, Issue 6779, pp. 778-82, 2000 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)", type "Antibodies"
Hosts Rabbit (145), Mouse (26), Sheep (4), Goat (1)
Reactivities Human (166), Mouse (Murine) (75), Rat (Rattus) (66), Cow (Bovine) (20), Pig (Porcine) (19), Sheep (Ovine) (15), Dog (Canine) (8), Chicken (7), Cat (Feline) (1), Hamster (1), Rabbit (1)
Applications Western Blotting (WB) (114), ELISA (65), Immunohistochemistry (IHC) (57), Immunofluorescence (IF) (50), Immunoprecipitation (IP) (36), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (29), Flow Cytometry (FACS) (3), Gel Shift (GS) (3), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (2), Electrophoretic Mobility-Shift Assay (EMSA) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Biotin (8), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr42 (15), pSer32, pSer36 (13), C-Term (8), AA 32-36 (6), N-Term (5), pTyr305 (5), AA 32-291 (4), pSer36,pSer32 (4), Ser32, Ser36 (3), AA 34-48 (2), Pser32,pSer36 (2), Ser32 (2), Ser32,Ser36 (2), Tyr42 (2), AA 130-143 (1), AA 32-47 (1), Internal Region (1), Ser23 (1), Sumoylation Site (1), Tyr181 (1), pSer32 (1), pSer32,pSer36 (1), pSer320 (1), pSer36 (1), pSer893 (1)