Protein Kinase, CGMP-Dependent, Type II (PRKG2) (C-Term) antibody

Antigen: Protein Kinase, CGMP-Dependent, Type II (PRKG2)

Synonyms: cGKII, PRKGR2, CGKII, Prkgr2, cGK-II, AW212535, MGC130517, GDPKII, MGC82580, PRKG2, prkg2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392537

Additional Information

Alternative name: cGKII

Gene ID: 5593

UniProt: Q13237

Immunogen: This cGKII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 717~746 amino acids from the C-terminal region of human cGKII.

Isotype: Ig

Description: Other names: cGMP-dependent protein kinase 2, Type II cGMP-dependent protein kinase, PRKGR2, PRKG2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This cGKII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 717~746 amino acids from the C-terminal region of human cGKII.

Molecular Weight: 87432 DA

Comments:

Background: cGKII is thought to play a key role in a diverse set of physiological pathway. cGKII may mediate intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Edentification of the pathway that mediates intestinal fluid secretion by E. coli STa has potential medical implications because STa causes traveler's diarrhea and about 50% of infant mortality in developing countries. Transfection experiments in human cells disclose that cGKII phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. Impaired differentiation of cultured KMI chondrocytes can be restored by silencing Sox9 by RNA interference. cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Immunohistochemistry~~1:50~100 Western Blot ~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of cGKII (arrow) using cGKII Antibody (C-term) (ABIN392537). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKG2 gene (Lane 2).

Western blot analysis of cGKII (arrow) using cGKII Antibody (C-term) (ABIN392537) in MC3T3 lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Fujii, Ogata, Takahashi et al.: "Expression of the human cGMP-dependent protein kinase II gene is lost upon introduction of SV40 T antigen or immortalization in human cells." in: FEBS letters, Vol. 375, Issue 3, pp. 263-7, 1996 (PubMed).

Orstavik, Solberg, Taskén et al.: "Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase." in: Biochemical and biophysical research communications, Vol. 220, Issue 3, pp. 759-65, 1996 (PubMed).

Gambaryan, Palmetshofer, Glazova et al.: "Inhibition of cGMP-dependent protein kinase II by its own splice isoform." in: Biochemical and biophysical research communications, Vol. 293, Issue 5, pp. 1438-44, 2002 (PubMed).

Vaandrager, Hogema, Edixhoven et al.: "Autophosphorylation of cGMP-dependent protein kinase type II." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28651-8, 2003 (PubMed).