P70 S6Kbeta (N-Term) antibody

Antigen: P70 S6Kbeta

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392548

Additional Information

Gene ID: 6199

UniProt: Q9UBS0

Immunogen: This p70 S6Kbeta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human p70 S6Kbeta Antibody.

Isotype: Ig

Description: Other names: Ribosomal protein S6 kinase beta-2, S6K2, 70 kDa ribosomal protein S6 kinase 2, p70-S6KB, p70 ribosomal S6 kinase beta, p70 S6Kbeta, p70 S6 kinase beta, S6K-beta, p70-beta, S6 kinase-related kinase, SRK, Serine/threonine-protein kinase 14 beta, RPS6KB2, S

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This p70 S6Kbeta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human p70 S6Kbeta Antibody.

Molecular Weight: 53483 DA

Comments:

Background: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B. Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50-100 Immunoprecipitation ~~ Not tested Flow cytometry ~~ Not tested

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Cell Cycle, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-p70 S6Kbeta Antibody (N-term) Pab (ABIN392548) in HepG2 cell line lysates (35ug/lane). RPS6KB2(arrow) was detected using the purified Pab (1:60 dilution).

Western blot analysis of anti-p70 S6Kbeta Antibody (N-term) Pab (ABIN392548) in 293 cell line lysates transiently transfected with the RPS6KB2 gene (2ug/lane). p70 S6Kbeta(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with p70 S6Kbeta Antibody (N-term)(ABIN392548), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Levy, Ronen, Bershadsky et al.: "Sustained induction of ERK, protein kinase B, and p70 S6 kinase regulates cell spreading and formation of F-actin microspikes upon ligation of integrins by galectin-8, a mammalian lectin." in: The Journal of biological chemistry, Vol. 278, Issue 16, pp. 14533-42, 2003 (PubMed).

Raught, Peiretti, Gingras et al.: "Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases." in: The EMBO journal, Vol. 23, Issue 8, pp. 1761-9, 2004 (PubMed).