PIP5K1A antibody (N-Term)

Catalog No. ABIN392593

Product Details anti-PIP5K1A Antibody

Target: PIP5K1A (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A))

Binding Specificity: AA 19-50, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIP5K1A antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PIP5K1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-50 amino acids from the N-terminal region of human PIP5K1A.

Clone: RB01727

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PIP5K1A antibody (ABIN392593)

Bolomini-Vittori, Montresor, Giagulli, Staunton, Rossi, Martinello, Constantin, Laudanna: "Regulation of conformer-specific activation of the integrin LFA-1 by a chemokine-triggered Rho signaling module." in: Nature immunology, Vol. 10, Issue 2, pp. 185-94, (2009) (PubMed).

Zhu, Zeisel: "Gene expression profiling in phosphatidylethanolamine N-methyltransferase knockout mice." in: Brain research. Molecular brain research, Vol. 134, Issue 2, pp. 239-55, (2005) (PubMed).

Target Details for PIP5K1A

Target: PIP5K1A (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A))

Alternative Name: PIP5K1A (PIP5K1A Products)

Background: Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.

Molecular Weight: 62633

Gene ID: 8394

NCBI Accession: NP_001129108, NP_001129109, NP_001129110, NP_003548

UniProt: Q99755

Pathways: PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Inositol Metabolic Process, DNA Replication, Cell-Cell Junction Organization, Synthesis of DNA