ATR (Center) antibody

Antigen: ATR

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN392606

Additional Information

Gene ID: 545

UniProt: Q13535

Immunogen: This ATR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149~178 amino acids from the center region of human ATR.

Isotype: Ig

Description: Other names: FRAP-related protein, Protein kinase ATR

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149~178 amino acids from the center region of human ATR.

Molecular Weight: 301449 DA

Comments:

Background: The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

ATR Antibody (L156) (ABIN392606) western blot analysis in uterine tumor cell line lysates (35ug/lane).This demonstrates the ATR antibody detected the ATR protein (arrow).

Publications

Product: Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).