Bromodomain Containing 1 (BRD1) (C-Term) antibody

Antigen: Bromodomain Containing 1 (BRD1)

Synonyms: BRL, BRPF1, BRPF2, DKFZp686F0325, AI316859, KIAA4191, MGC164733, mKIAA4191, 1110059H06Rik, MGC80898, BRD1, im:7138929, si:dkey-164m14.6, LOC733743, MGC146307, BDNF

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392608

Additional Information

Alternative name: BRD1

Gene ID: 23774

UniProt: O95696

Immunogen: This BRD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1029~1058 amino acids from the C-terminal region of human BRD1.

Isotype: Ig

Description: Other names: Bromodomain-containing protein 1, BR140-like protein

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BRD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1029~1058 amino acids from the C-terminal region of human BRD1.

Molecular Weight: 119519 DA

Comments:

Background: This gene encodes a protein of unknown function. The protein contains a bromodomain, a sequence motif often found in transcriptional coactivators, and localizes to the nucleus in testis and several other cell types.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

BRD1 Antibody (L1042) (ABIN392608) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the BRD1 antibody detected the BRD1 protein (arrow).

Publications

Product: Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

McCullagh, Chaplin, Meerabux et al.: "The cloning, mapping and expression of a novel gene, BRL, related to the AF10 leukaemia gene." in: Oncogene, Vol. 18, Issue 52, pp. 7442-52, 2000 (PubMed).