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Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (Center) antibody
Alternatives Western Blotting (WB), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This BUB1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268~298 amino acids from the Center region of human BUB1B.|
|Description||Other names: Mitotic checkpoint serine/threonine-protein kinase BUB1, hBub1, BUB1A|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This BUB1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268~298 amino acids from the Center region of human BUB1B.|
|Molecular Weight||119517 DA|
Background: BUB1B, a member of the Ser/Thr protein kinase family, is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. It can interact with BUB3, CENP-F, CENP-E and mitosin, and can be localized to nuclear kinetochores. This protein is highly expressed in thymus and spleen. The CD1 domain directs kinetochore localization and binding to BUB3. The protein possesses a cyclin destruction box sequence, which targets protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase. Defects in BUB1B are associated with tumor formation.
|Synonyms||SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta, Bub1b, BUB1B, bub1b, Bub1a, C80208, D2Xrf87, AL022991, AU045529, MGC36092, BUB1, BUB1-like, BubR1, CG14030/Bub1, DmelCG14030, CG14030, 31/13, BcDNA:LD23835, CG 7838, DmBUB1, bubR1, dBUB1, l(2)k03113, l(2)k06109, DmelCG7838, CG7838|
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western Blot~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
|Western blot analysis of BUB1B(arrow) using rabbit polyclonal BUB1B Antibody (Center) (ABIN392628). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).|
Cahill, Lengauer, Yu et al.: "Mutations of mitotic checkpoint genes in human cancers." in: Nature, Vol. 392, Issue 6673, pp. 300-3, 1998 (PubMed).
Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, 1998 (PubMed).
Davenport, Fernandes, Harris et al.: "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner." in: Genomics, Vol. 55, Issue 1, pp. 113-7, 1999 (PubMed).
Cahill, da Costa, Carson-Walter et al.: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999 (PubMed).
|Hosts||Rabbit (37), Mouse (24), Sheep (3)|
|Reactivities||Human (61), Mouse (Murine) (24), Cow (Bovine) (19), Monkey (3), Dog (Canine) (2), Rat (Rattus) (2), Cat (Feline) (1), Chicken (1)|
|Applications||Western Blotting (WB) (48), Immunofluorescence (IF) (34), ELISA (22), Immunohistochemistry (IHC) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (8), Flow Cytometry (FACS) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Proximity Ligation Assay (PLA) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (9), AA 1-350 (2), Internal Region (2), AA 1-50 (1), AA 350-400 (1), C-Term (1), Center (1)|