Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (N-Term) antibody

Antigen: Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B)

Synonyms: SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta, Bub1b, BUB1B, bub1b, Bub1a, C80208, D2Xrf87, AL022991, AU045529, MGC36092, BUB1, BUB1-like, BubR1, CG14030/Bub1, DmelCG14030, CG14030, 31/13, BcDNA:LD23835, CG 7838, DmBUB1, bubR1, dBUB1, l(2)k03113, l(2)k06109, DmelCG7838, CG7838

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392629

Additional Information

Alternative name: BUB1B

Gene ID: 701

UniProt: O60566

Immunogen: This BUB1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99~128 amino acids from the N-terminal region of human BUB1B.

Isotype: Ig

Description: Other names: Mitotic checkpoint serine/threonine-protein kinase BUB1, hBub1, BUB1A

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BUB1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99~128 amino acids from the N-terminal region of human BUB1B.

Molecular Weight: 119517 DA

Comments:

Background: BUB1B, a member of the Ser/Thr protein kinase family, is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. It can interact with BUB3, CENP-F, CENP-E and mitosin, and can be localized to nuclear kinetochores. This protein is highly expressed in thymus and spleen. The CD1 domain directs kinetochore localization and binding to BUB3. The protein possesses a cyclin destruction box sequence, which targets protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase. Defects in BUB1B are associated with tumor formation.

Synonyms: SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta, Bub1b, BUB1B, bub1b, Bub1a, C80208, D2Xrf87, AL022991, AU045529, MGC36092, BUB1, BUB1-like, BubR1, CG14030/Bub1, DmelCG14030, CG14030, 31/13, BcDNA:LD23835, CG 7838, DmBUB1, bubR1, dBUB1, l(2)k03113, l(2)k06109, DmelCG7838, CG7838

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blot~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

(LEFT)Western blot analysis of BUB1B Antibody (N-term) (ABIN392629) in Hela cell line lysates (35ug/lane).BUB1B (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of BUB1B(arrow) using rabbit polyclonal BUB1B Antibody (N-term) (ABIN392629). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).

Publications

Product: Cahill, Lengauer, Yu et al.: "Mutations of mitotic checkpoint genes in human cancers." in: Nature, Vol. 392, Issue 6673, pp. 300-3, 1998 (PubMed).

Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, 1998 (PubMed).

Davenport, Fernandes, Harris et al.: "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner." in: Genomics, Vol. 55, Issue 1, pp. 113-7, 1999 (PubMed).

Cahill, da Costa, Carson-Walter et al.: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999 (PubMed).