|+1 404 474 4654|
|+1 888 205 9894 (TF)|
NIMA (Never in Mitosis Gene A)-Related Kinase 6 (NEK6) (N-Term) antibody
Alternatives Western Blotting (WB), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NEK6.|
|Description||Other names: Serine/threonine-protein kinase Nek6, NIMA-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This NEK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42~71 amino acids from the N-terminal region of human NEK6.|
Background: NEK6 is a serine/threonine kinase that controls initiation of mitosis. NEK6 is activated during M phase. It is required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis.
|Application Notes||The suggested dilution will be: ELISA 1:1,000 Western blotting 1:100~500 Flow cytometry|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Research Area||Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
|Western blot analysis of NEK6 (arrow) using rabbit polyclonal NEK6 Antibody (N-term)(ABIN392652). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK6 gene (Lane 2) (Origene Technologies).|
Lizcano, Deak, Morrice et al.: "Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 27839-49, 2002 (PubMed).
Hashimoto, Akita, Hibino et al.: "Identification and characterization of Nek6 protein kinase, a potential human homolog of NIMA histone H3 kinase." in: Biochemical and biophysical research communications, Vol. 293, Issue 2, pp. 753-8, 2002 (PubMed).
Belham, Roig, Caldwell et al.: "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases." in: The Journal of biological chemistry, Vol. 278, Issue 37, pp. 34897-909, 2003 (PubMed).
|Hosts||Rabbit (32), Mouse (4)|
|Reactivities||Human (35), Mouse (Murine) (25), Rat (Rattus) (23), Chicken (20), Cow (Bovine) (20), Dog (Canine) (20), Pig (Porcine) (19), Horse (Equine) (18), Cat (Feline) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Immunofluorescence (IF) (19), Western Blotting (WB) (17), ELISA (12), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) pro (IHC (p)+) (2), Immunoprecipitation (IP) (2), Dot Blot (DB) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Transcript Variant 2 (4), N-Term (3), C-Term (2), pSer121 (1)|