Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG)

Synonyms: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, IKK-gamma, IKK[g], AI848108, AI851264, AW124339, 1110037D23Rik, Nemo, nemo, fj33c07, wu:fj33c07, zgc:113492, MGC97885, IKBKG, IKBKG_1, IKBKG_2, DmIKK-gamma, DmIKKgamma, IKKg, IKKgamma, Kenny, Key, dIKK, dIKK-gamma, DmelCG16910, CG16910

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392700

Additional Information

Alternative name: IKK gamma

Gene ID: 8517

UniProt: NEMO_HUMAN

Immunogen: This IKK gamma antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length sequence of human IKK gamma.

Isotype: Ig

Description: Other names: NF-kappaB essential modulator, NEMO, NF-kappaB essential modifier, Inhibitor of nuclear factor kappa-B kinase gamma subunit, IkB kinase gamma subunit, I-kappa-B kinase gamma, IKKG, IkB kinase-associated protein 1, IKKAP1, FIP-3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IKK gamma antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length sequence of human IKK gamma.

Molecular Weight: 48197 DA

Comments:

Background: IKK gamma is a regulatory subunit part of the IKK-signalosome complex activation. It is also considered to be a mediator for TAX activation of NF-kappa-B. This protein could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Defects in IKBKG are the cause of anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID). EDA-ID is a X-linked recessive disorder characterized by absence of sweat glands, sparse scalp hair, rare conical teeth and immunological abnormalities resulting in severe infectious diseases. Defects in IKBKG are the cause of familial incontinentia pigmenti type II (IP2), an X-linked dominant disease causing death in male fetuses. In heterozygous female, it is characterized by disturbance of skin pigmentation sometimes associated with a variety of malformations of the eye, nails, teeth, skeleton, heart, and central nervous system.

Synonyms: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, IKK-gamma, IKK[g], AI848108, AI851264, AW124339, 1110037D23Rik, Nemo, nemo, fj33c07, wu:fj33c07, zgc:113492, MGC97885, IKBKG, IKBKG_1, IKBKG_2, DmIKK-gamma, DmIKKgamma, IKKg, IKKgamma, Kenny, Key, dIKK, dIKK-gamma, DmelCG16910, CG16910

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of IKK gamma Antibody (ABIN392700) in HepG2 cell line lysates (35ug/lane). IKBKG (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with IKK gamma Antibody (ABIN392700), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Chariot, Leonardi, Muller et al.: "Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 37029-36, 2002 (PubMed).

Tegethoff, Behlke, Scheidereit: "Tetrameric oligomerization of IkappaB kinase gamma (IKKgamma) is obligatory for IKK complex activity and NF-kappaB activation." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2029-41, 2003 (PubMed).

Tang, Wang, Xiong et al.: "A role for NF-kappaB essential modifier/IkappaB kinase-gamma (NEMO/IKKgamma) ubiquitination in the activation of the IkappaB kinase complex by tumor necrosis factor-alpha." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37297-305, 2003 (PubMed).

Mauro, Vito, Mellone et al.: "Role of the adaptor protein CIKS in the activation of the IKK complex." in: Biochemical and biophysical research communications, Vol. 309, Issue 1, pp. 84-90, 2003 (PubMed).