PFKP antibody (C-Term)

Catalog No. ABIN392744

Product Details anti-PFKP Antibody

Target: PFKP (phosphofructokinase, Platelet (PFKP))

Binding Specificity: AA 754-784, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PFKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-784 amino acids from the C-terminal region of human PFKP.

Clone: RB03900

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:8000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PFKP antibody (ABIN392744)

Bao, Mukai, Hishiki, Kubo, Ohmura, Sugiura, Matsuura, Nagahata, Hayakawa, Yamamoto, Fukuda, Saya, Suematsu, Minamishima: "Energy management by enhanced glycolysis in G1-phase in human colon cancer cells in vitro and in vivo." in: Molecular cancer research : MCR, Vol. 11, Issue 9, pp. 973-85, (2013) (PubMed).

Yalcin, Clem, Simmons, Lane, Nelson, Clem, Brock, Siow, Wattenberg, Telang, Chesney: "Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases." in: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24223-32, (2009) (PubMed).

Moeller, Dumitrescu, Refetoff: "Cytosolic action of thyroid hormone leads to induction of hypoxia-inducible factor-1alpha and glycolytic genes." in: Molecular endocrinology (Baltimore, Md.), Vol. 19, Issue 12, pp. 2955-63, (2005) (PubMed).

Target Details for PFKP

Target: PFKP (phosphofructokinase, Platelet (PFKP))

Alternative Name: PFKP (PFKP Products)

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Molecular Weight: 85596

Gene ID: 5214

NCBI Accession: NP_001229268, NP_002618

UniProt: Q01813

Pathways: Warburg Effect