Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (C-Term) antibody

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE)

Synonyms: IKKE, IKKI, IKK-i, KIAA0151, MGC125294, MGC125295, MGC125297, zgc:100844, IKBKE, MGC137373, MGC130907

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392751

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: IKK epsilon (IKKE) (C-term)

Gene ID: 9641

Swiss-Prot: Q14164

Immunogen: This IKKE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 687~716 amino acids from the C-terminal region of human IKKE.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Inhibitor of nuclear factor kappa-B kinase epsilon subunit, I kappa-B kinase epsilon, IkBKE, IKK-epsilon, IKK-E, Inducible I kappa-B kinase, IKK-i

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human IKKE.

Molecular Weight: 80462 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

The anti-IKKE Pab (ABIN392751) is used in Western blot to detect IKKE in 293 cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IKKE Antibody (C-term) (ABIN392751), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.