|+1 404 474 4654|
|+1 888 205 9894 (TF)|
tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) (N-Term) antibody
|Synonyms||HS1, GW128, KCIP-1, 1300003C17Rik, ywhab, MGC53093, MGC78918, 14-3-3beta, hs1, gw128, kcip-1, YWHAB, 1433b, DKFZp459L1720|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||YWHAB (14-3-3 protein beta/alpha)|
|Immunogen||This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~93 amino acids from the N-terminal region of human YWHAB.|
|Description||Other names: 14-3-3 protein beta/alpha, Protein kinase C inhibitor protein-1, KCIP-1, Protein 1054|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~93 amino acids from the N-terminal region of human YWHAB.|
|Molecular Weight||27951 DA|
Background: YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
Shumway, Li, Xiong: "14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin." in: The Journal of biological chemistry, Vol. 278, Issue 4, pp. 2089-92, 2003 (PubMed).
Parvaresch, Yesilkaya, Baer et al.: "14-3-3 binding to the IGF-1 receptor is mediated by serine autophosphorylation." in: FEBS letters, Vol. 532, Issue 3, pp. 357-62, 2002 (PubMed).
Li, Inoki, Vacratsis et al.: "The p38 and MK2 kinase cascade phosphorylates tuberin, the tuberous sclerosis 2 gene product, and enhances its interaction with 14-3-3." in: The Journal of biological chemistry, Vol. 278, Issue 16, pp. 13663-71, 2003 (PubMed).
Cavet, Lehoux, Berk: "14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18376-83, 2003 (PubMed).
Komori, Ishizawa, Arai et al.: "Immunoexpression of 14-3-3 proteins in glial cytoplasmic inclusions of multiple system atrophy." in: Acta neuropathologica, Vol. 106, Issue 1, pp. 66-70, 2003 (PubMed).
|Hosts||Rabbit (36), Mouse (7)|
|Reactivities||Human (42), Mouse (Murine) (23), Rat (Rattus) (23), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Cow (Bovine) (3), Zebrafish (Danio rerio) (3), Chicken (2), Dog (Canine) (2), Cat (Feline) (1), Primate (1), Rabbit (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (38), ELISA (23), Immunofluorescence (IF) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (5), Immunocytochemistry (ICC) (4)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), FITC (1), HRP (1), PE,Cy5.5 (1)|
|Epitopes||C-Term (7), N-Term (2), AA 1-246 (1), Center (1)|