SAD1 (BRSK1) (N-Term), (AA 108-139) antibody

Details for Product No. ABIN392801
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Antigen
Epitope
N-Term, AA 108-139
Reactivity
Human
Host
Rabbit
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN392801
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Immunogen This SAD1 (BRSK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-139 AA from the N-terminal region of human SAD1 (BRSK1).
Clone RB5413
Isotype Ig
Specificity This SAD1 (BRSK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BRSK1.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background BRSK1 may be involved as a checkpoint kinase in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. This protein phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. BRSK1 is partitioned between cytoplasmic and nuclear locations in the absence of DNA damage, but translocates to the nucleus in response to Uv- or MMS-induced DNA damage. BRSK1 shares significant homology with the fission yeast Cdr2, a mitosis-regulatory kinase, and Caenorhabditis elegans SAD1, a neuronal cell polarity regulator. The BRSK1 transcript is expressed ubiquitously with the highest levels of expression in brain and testis.
Synonyms: BR serine/threonine-protein kinase 1, SAD1 kinase, SAD1A , BRSK1
Molecular Weight 85086 DA
Gene ID 84446
NCBI Accession NP_115806
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SAD1 (BRSK1) (N-Term), (AA 108-139) antibody Western blot analysis of anti-KIAA181 Pab (ABIN392801) in mouse liver tissue lysate (35 µg/lane). KIAA181(arrow) was detected using the purified polyclonal antibody (1:60 dilution).
Background publications Lizcano, Göransson, Toth et al.: "LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1." in: The EMBO journal, Vol. 23, Issue 4, pp. 833-43, 2004 (PubMed).

Lu, Niida, Nakanishi: "Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function." in: The Journal of biological chemistry, Vol. 279, Issue 30, pp. 31164-70, 2004 (PubMed).

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