Protein tyrosine Phosphatase, Receptor Type, R (PTPRR) (N-Term) antibody

Antigen: Protein tyrosine Phosphatase, Receptor Type, R (PTPRR)

Synonyms: Pcptp1, ec-ptp, pcptp1, ptp-sl, ptpbr7, PTPRR, PCPTP1, PTPRQ, DKFZp459C2431

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392828

Additional Information

Alternative name: PCPTP1

Gene ID: 5801

UniProt: Q15256

Immunogen: This PCPTP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242~272 amino acids from the N-terminal region of human PCPTP1.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PCPTP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242~272 amino acids from the N-terminal region of human PCPTP1.

Molecular Weight: 73862 DA

Comments:

Background: PCPTP1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domains, and thus represents a receptor-type PTP. The similar gene for this protein predominately expressed in mouse brain was found to associate with, and thus regulate the activity and cellular localization of MAP kinases. The rat counterpart of the gene for this protein was reported to be regulated by the nerve growth factor, which suggested a function in neuronal growth and differentiation.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Cell Cycle, Neurology

Restrictions: For Research Use only

Images

The anti-PCPTP1 Pab (ABIN392828) is used in Western blot to detect PCPTP1 in placenta tissue lysate.

Formalin-fixed and paraffin-embedded human brain tissue reacted with hPCPTP1-Y249, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Ogata, Sawada, Fujino et al.: "cDNA cloning and characterization of a novel receptor-type protein tyrosine phosphatase expressed predominantly in the brain." in: The Journal of biological chemistry, Vol. 270, Issue 5, pp. 2337-43, 1995 (PubMed).

Blanco-Aparicio, Torres, Pulido: "A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1129-36, 2000 (PubMed).