Protein tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2) (C-Term,Lys369) antibody

Antigen: Protein tyrosine Phosphatase, Non-Receptor Type 2 (PTPN2)

Binding Site: C-Term,Lys369

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392844

Additional Information

Alternative name: TCPTP

Gene ID: 5771

UniProt: P17706

Immunogen: This TCPTP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361~390 amino acids from the C-terminal region of human TCPTP

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TCPTP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361~390 amino acids from the C-terminal region of human TCPTP

Molecular Weight: 48528 DA

Comments:

Background: TCPTP is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Three alternatively spliced variants of the gene, which encode isoforms differing at their extreme C-termini, have been described. The different C-termini are thought to determine the substrate specificity, as well as the cellular localization of the isoforms.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-TCPTP Pab (ABIN392844) in HL60 cell line lysate (35ug/lane). TCPTP(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with TCPTP antibody (C-term K369), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Zhu, Mustelin, David: "Arginine methylation of STAT1 regulates its dephosphorylation by T cell protein tyrosine phosphatase." in: The Journal of biological chemistry, Vol. 277, Issue 39, pp. 35787-90, 2002 (PubMed).

Dupuis, De Jesús Ibarra-Sánchez, Tremblay et al.: "Gr-1+ myeloid cells lacking T cell protein tyrosine phosphatase inhibit lymphocyte proliferation by an IFN-gamma- and nitric oxide-dependent mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 726-32, 2003 (PubMed).