Dual Specificity Phosphatase 3 (DUSP3) antibody

Antigen: Dual Specificity Phosphatase 3 (DUSP3)

Synonyms: VHR, T-DSP11, 2210015O03Rik, 5031436O03Rik, DUSP3, MGC189429, RGD1560049, si:bz18k17.3, si:rp71-18k17.3, MGC152292

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392858

Additional Information

Alternative name: DUSP3

Gene ID: 1845

UniProt: P51452

Immunogen: This DUSP3 antibody is generated from rabbits immunized with a 6xHIS-tagged recombinant protein encoding aa 1~185 of human VHR.

Isotype: Ig

Description: Other names: Dual specificity protein phosphatase 3, Dual specificity protein phosphatase VHR, VHR

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DUSP3 antibody is generated from rabbits immunized with a 6xHIS-tagged recombinant protein encoding aa 1~185 of human VHR.

Molecular Weight: 20478 DA

Comments:

Background: Dual specificity protein phosphatases (DSPs) regulate mitogenic signal transduction and control the cell cycle. The crystal structure of a human DSP shows a shallow active site pocket in VHR allows for the hydrolysis of phosphorylated serine, threonine, or tyrosine protein residues, whereas the deeper active site of protein tyrosine phosphatases (PTPs) restricts substrate specificity to only phosphotyrosine. Positively charged crevices near the active site may explain the enzyme's preference for substrates with two phosphorylated residues. The VHR structure defines a conserved structural scaffold for both DSPs and PTPs. A ``recognition region,'' connecting helix alpha 1 to strand beta 1, may determine differences in substrate specificity between VHR, the PTPs, and other DSPs.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-DUSP3 Pab (ABIN392858) in HepG2 tissue lysate (35ug/lane). DUSP3(arrow) was detected using the purified Pab.

Publications

Product: Ishibashi, Bottaro, Chan et al.: "Expression cloning of a human dual-specificity phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 24, pp. 12170-4, 1993 (PubMed).

Yuvaniyama, Denu, Dixon et al.: "Crystal structure of the dual specificity protein phosphatase VHR." in: Science (New York, N.Y.), Vol. 272, Issue 5266, pp. 1328-31, 1996 (PubMed).