|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Dual Specificity Phosphatase 16 (DUSP16) (N-Term) antibody
|Synonyms||DUSP16, MKP7, Mkpm, MKP-7, AW558566, D6Ertd213e, 3830417M17Rik, KIAA1700, MGC129701, MGC129702, dusp16, wu:fi04g09, mkp7, mkp-7|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This DUSP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12~41 amino acids from the N-terminal region of human DUSP16.|
|Description||Other names: Dual specificity protein phosphatase 16, Mitogen-activated protein kinase phosphatase 7, MAP kinase phosphatase 7, MKP-7,|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This DUSP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12~41 amino acids from the N-terminal region of human DUSP16.|
|Molecular Weight||73102 DA|
Background: DUSP16 is involved in the inactivation of MAP kinases. The activation of mitogen-activated protein kinase (MAPK) cascades transduces various extracellular signals to the nucleus to induce gene expression, cell proliferation, differentiation, cell cycle arrest, and apoptosis. For full activation of MAPKs, dual-specificity kinases phosphorylate both threonine and tyrosine residues in MAPK TXY motifs. MKPs are dual-specificity phosphatases that dephosphorylate the TXY motif, thereby negatively regulating MAPK activity.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
Masuda, Shima, Watanabe et al.: "MKP-7, a novel mitogen-activated protein kinase phosphatase, functions as a shuttle protein." in: The Journal of biological chemistry, Vol. 276, Issue 42, pp. 39002-11, 2001 (PubMed).
Willoughby, Perkins, Collins et al.: "The JNK-interacting protein-1 scaffold protein targets MAPK phosphatase-7 to dephosphorylate JNK." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10731-6, 2003 (PubMed).
Masuda, Shima, Katagiri et al.: "Activation of ERK induces phosphorylation of MAPK phosphatase-7, a JNK specific phosphatase, at Ser-446." in: The Journal of biological chemistry, Vol. 278, Issue 34, pp. 32448-56, 2003 (PubMed).
Hoornaert, Marynen, Goris et al.: "MAPK phosphatase DUSP16/MKP-7, a candidate tumor suppressor for chromosome region 12p12-13, reduces BCR-ABL-induced transformation." in: Oncogene, Vol. 22, Issue 49, pp. 7728-36, 2003 (PubMed).
Katagiri, Masuda, Urano et al.: "Phosphorylation of Ser-446 determines stability of MKP-7." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 14716-22, 2005 (PubMed).
|Hosts||Rabbit (12), Goat (6)|
|Reactivities||Human (17), Mouse (Murine) (11), Cow (Bovine) (7), Monkey (4), Rat (Rattus) (4), Chimpanzee (3), Dog (Canine) (3), Chicken (2), Horse (Equine) (1), Zebrafish (1), Zebrafish (Danio rerio) (1)|
|Applications||ELISA (11), Western Blotting (WB) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunofluorescence (IF) (2), Immunohistochemistry (IHC) (2), Dot Blot (DB) (1)|
|Epitopes||C-Term (3), N-Term (2), AA 55-70 (1), AA 600-650 (1)|