Dual Specificity Phosphatase 16 (DUSP16) (C-Term) antibody

Antigen: Dual Specificity Phosphatase 16 (DUSP16)

Synonyms: DUSP16, MKP7, Mkpm, MKP-7, AW558566, D6Ertd213e, 3830417M17Rik, KIAA1700, MGC129701, MGC129702, dusp16, wu:fi04g09, mkp7, mkp-7

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392882

Additional Information

Alternative name: DUSP16

Gene ID: 80824

UniProt: Q9BY84

Immunogen: This DUSP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 598~627 amino acids from the C-terminal region of human DUSP16.

Isotype: Ig

Description: Other names: Dual specificity protein phosphatase 16, Mitogen-activated protein kinase phosphatase 7, MAP kinase phosphatase 7, MKP-7,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DUSP16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 598~627 amino acids from the C-terminal region of human DUSP16.

Molecular Weight: 73102 DA

Comments:

Background: DUSP16 is involved in the inactivation of MAP kinases. The activation of mitogen-activated protein kinase (MAPK) cascades transduces various extracellular signals to the nucleus to induce gene expression, cell proliferation, differentiation, cell cycle arrest, and apoptosis. For full activation of MAPKs, dual-specificity kinases phosphorylate both threonine and tyrosine residues in MAPK TXY motifs. MKPs are dual-specificity phosphatases that dephosphorylate the TXY motif, thereby negatively regulating MAPK activity.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-DUSP16 Pab(ABIN392882) in 293 cell line lysate (35ug/lane). DUSP16(arrow) was detected using the purified Pab (1:50 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Masuda, Shima, Watanabe et al.: "MKP-7, a novel mitogen-activated protein kinase phosphatase, functions as a shuttle protein." in: The Journal of biological chemistry, Vol. 276, Issue 42, pp. 39002-11, 2001 (PubMed).

Willoughby, Perkins, Collins et al.: "The JNK-interacting protein-1 scaffold protein targets MAPK phosphatase-7 to dephosphorylate JNK." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10731-6, 2003 (PubMed).

Masuda, Shima, Katagiri et al.: "Activation of ERK induces phosphorylation of MAPK phosphatase-7, a JNK specific phosphatase, at Ser-446." in: The Journal of biological chemistry, Vol. 278, Issue 34, pp. 32448-56, 2003 (PubMed).

Hoornaert, Marynen, Goris et al.: "MAPK phosphatase DUSP16/MKP-7, a candidate tumor suppressor for chromosome region 12p12-13, reduces BCR-ABL-induced transformation." in: Oncogene, Vol. 22, Issue 49, pp. 7728-36, 2003 (PubMed).

Katagiri, Masuda, Urano et al.: "Phosphorylation of Ser-446 determines stability of MKP-7." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 14716-22, 2005 (PubMed).