Calcineurin (PPP3CA) (C-Term) antibody

Antigen: Calcineurin (PPP3CA)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392892

Additional Information

Gene ID: 5494

UniProt: P35813

Immunogen: This PPP3CA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PPP3CA.

Isotype: Ig

Description: Other names: Serine/threonine protein phosphatase 2B , Calmodulin-dependent calcineurin A subunit, alpha isoform, CAM-PRP catalytic subunit, CALNA, CNA

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PPP3CA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PPP3CA.

Molecular Weight: 58688 DA

Comments:

Background: PPP3CA is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-PPP3CA Pab(ABIN392892) in mouse liver tissue lysate (35ug/lane). PPP3CA(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Cheng, Ross, Kaldis et al.: "Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases." in: Genes & development, Vol. 13, Issue 22, pp. 2946-57, 2000 (PubMed).

Ofek, Ben-Meir, Kariv-Inbal et al.: "Cell cycle regulation and p53 activation by protein phosphatase 2C alpha." in: The Journal of biological chemistry, Vol. 278, Issue 16, pp. 14299-305, 2003 (PubMed).

Matsuda, Suzuki, Honda et al.: "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." in: Oncogene, Vol. 22, Issue 21, pp. 3307-18, 2003 (PubMed).

Flajolet, Rakhilin, Wang et al.: "Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 26, pp. 16006-11, 2003 (PubMed).

Yoshizaki, Maegawa, Egawa et al.: "Protein phosphatase-2C alpha as a positive regulator of insulin sensitivity through direct activation of phosphatidylinositol 3-kinase in 3T3-L1 adipocytes." in: The Journal of biological chemistry, Vol. 279, Issue 21, pp. 22715-26, 2004 (PubMed).