Keratin 7/17 antibody

Catalog No. ABIN392948

Product Details anti-Keratin 7/17 Antibody

Target: Keratin 7/17 (KRT7/17)

Reactivity: Human, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 7/17 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a 54kD type II basic cytokeratin, and 46kD type I acidic cytokeratin respectively. Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for diagnosis and characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7. Reported as suitable for use in immunocytochemistry.

No Cross-Reactivity: Mouse (Murine), Rabbit, Rat (Rattus)

Purification: Affinity purified

Immunogen: HeLa cell cytoskeleton preparation.

Clone: C-46

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (20 - 30 μg/mL), IP, WB (2 - 3 μg/mL)

Usage: Western Blot: LS-C58267 detects bands of approximately 54kD and 46kD in HeLa cytoskeleton cell lysates under reducing conditions. Immunohistology: This product requires protein digestion pre-treatment of paraffin sections using pepsin prior to staining.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for Keratin 7/17

Target: Keratin 7/17 (KRT7/17)

Alternative Name: Cytokeratin 7+17 (KRT7/17 Products)

Synonyms: KRT7 antibody, CK7 antibody, keratin 7 antibody, KRT7 antibody

Background: This antibody detects: Cytokeratin 7+17