Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10) antibody

Antigen: Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10)

Synonyms: MCH4, ALPS2, FLICE2, xCaspase-10, mch4, alps2, flice2, MGC107844, caspace-10, caspase-10, xCaspace-10, LOC100101580

Clonality: Monoclonal (1D8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN393206

Additional Information

Alternative name: CASP10

Gene ID: 843

UniProt: NP_116756

Immunogen: CASP10 (NP_116756, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1D8

Description: Other names: ALPS2, FLICE2, MCH4 caspase 10, apoptosis-related cysteine protease

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CASP10 (NP_116756, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 58951 DA

Comments:

Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged CASP10 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Oh, Kim, Ahn et al.: "Mutational analysis of CASP10 gene in colon, breast, lung and hepatocellular carcinomas." in: Pathology, Vol. 42, Issue 1, pp. 73-6, 2009 (PubMed).

Kim, Park, Kim et al.: "Mutational analysis of caspase genes in prostate carcinomas." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 118, Issue 4, pp. 308-12, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Ho-Pun-Cheung, Assenat, Bascoul-Mollevi et al.: "A large-scale candidate gene approach identifies SNPs in SOD2 and IL13 as predictive markers of response to preoperative chemoradiation in rectal cancer." in: The pharmacogenomics journal, Vol. 11, Issue 6, pp. 437-43, 2011 (PubMed).

Lee, Choi, Choi et al.: "Polymorphisms in the caspase genes and the risk of lung cancer." in: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, Vol. 5, Issue 8, pp. 1152-8, 2010 (PubMed).