Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) antibody

Antigen: Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST)

Clonality: Monoclonal (4A4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393214

Additional Information

Alternative name: IL6ST

Gene ID: 3572

UniProt: NP_002175

Immunogen: IL6ST (NP_002175, 23 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4A4

Description: Other names: CD130, CDw130, GP130, GP130-RAPS, IL6R-beta interleukin 6 signal transducer (gp130, oncostatin M receptor)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: IL6ST (NP_002175, 23 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 103523 DA

Comments:

Background: The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Structure

Restrictions: For Research Use only

Images

IL6ST monoclonal antibody (M02), clone 4A4. Western Blot analysis of IL6ST expression in HepG2 ( Cat # L019V1 ).

Publications

Product: Xu, Kershaw, Luo et al.: "Crystal structure of the entire ectodomain of gp130: insights into the molecular assembly of the tall cytokine receptor complexes." in: The Journal of biological chemistry, Vol. 285, Issue 28, pp. 21214-8, 2010 (PubMed).

Larsen, Hansen, Møller et al.: "Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer." in: Molecular and cellular biology, Vol. 30, Issue 17, pp. 4175-87, 2010 (PubMed).

Hilfiker-Kleiner, Shukla, Klein et al.: "Continuous glycoprotein-130-mediated signal transducer and activator of transcription-3 activation promotes inflammation, left ventricular rupture, and adverse outcome in subacute myocardial infarction." in: Circulation, Vol. 122, Issue 2, pp. 145-55, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).