Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14) (AA 133-243) antibody

Details for Product No. ABIN393215
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Antigen
Synonyms CASP14, MICE, mini-ICE
Epitope
AA 133-243
(14), (9), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(63), (40), (37), (12), (1), (1)
Host
Mouse
(62), (5), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunofluorescence (IF), Western Blotting (WB)
(49), (24), (19), (17), (14), (7), (5), (5), (3), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393215
450.00 $
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Immunogen CASP14 (NP_036246, 133 a.a. ~ 243 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4C9
Isotype IgG2a kappa
Specificity CASP14 (NP_036246, 133 a.a. ~ 243 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CASP14
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 27680 DA
Gene ID 12406
UniProt NP_036246
Research Area Apoptosis/Necrosis, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14) (AA 133-243) antibody Immunofluorescence of to CASP14 on HeLa cell . CASP14 monoclonal antibody (M01), clone 4C9 Western Blot analysis of CASP14 expression in MCF-7.
anti-Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14) (AA 133-243) antibody (2) anti-Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14) (AA 133-243) antibody (Image 2)
Background publications White, Declercq, Arfuso et al.: "Function of caspase-14 in trophoblast differentiation." in: Reproductive biology and endocrinology : RB&E, Vol. 7, pp. 98, 2009 (PubMed).

Hosgood, Zhang, Shen et al.: "Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity." in: Occupational and environmental medicine, Vol. 66, Issue 12, pp. 848-53, 2009 (PubMed).

Hibino, Fujita, Tsuji et al.: "Purification and characterization of active caspase-14 from human epidermis and development of the cleavage site-directed antibody." in: Journal of cellular biochemistry, Vol. 109, Issue 3, pp. 487-97, 2010 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Kim, Park, Kim et al.: "Mutational analysis of caspase genes in prostate carcinomas." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 118, Issue 4, pp. 308-12, 2010 (PubMed).

Validation Images
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