Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14) antibody

Antigen: Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14)

Synonyms: MICE, mini-ICE, MGC123948, MGC123949, MGC119078, MGC119079, CASP14, LOC100101622

Clonality: Monoclonal (4C9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393215

Additional Information

Alternative name: CASP14

Gene ID: 23581

UniProt: NP_036246

Immunogen: CASP14 (NP_036246, 133 a.a. ~ 243 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4C9

Description: Other names: MGC119078, MGC119079 caspase 14, apoptosis-related cysteine peptidase

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CASP14 (NP_036246, 133 a.a. ~ 243 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 27680 DA

Comments:

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of to CASP14 ( Cat# AT1403a ) on HeLa cell . CASP14 monoclonal antibody (M01), clone 4C9 Western Blot analysis of CASP14 expression in MCF-7 ( Cat # L046V1 ).

anti-Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14) antibody (Image 2)

Publications

Product: White, Declercq, Arfuso et al.: "Function of caspase-14 in trophoblast differentiation." in: Reproductive biology and endocrinology : RB&E, Vol. 7, pp. 98, 2009 (PubMed).

Hosgood, Zhang, Shen et al.: "Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity." in: Occupational and environmental medicine, Vol. 66, Issue 12, pp. 848-53, 2009 (PubMed).

Hibino, Fujita, Tsuji et al.: "Purification and characterization of active caspase-14 from human epidermis and development of the cleavage site-directed antibody." in: Journal of cellular biochemistry, Vol. 109, Issue 3, pp. 487-97, 2010 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Kim, Park, Kim et al.: "Mutational analysis of caspase genes in prostate carcinomas." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 118, Issue 4, pp. 308-12, 2010 (PubMed).