Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody

Antigen: Second Mitochondria-Derived Activator of Caspase (DIABLO)

Synonyms: SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049, smac, smac3, diablo-s, XSmac, diablo

Clonality: Monoclonal (4F9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393216

Additional Information

Alternative name: DIABLO

Gene ID: 56616

Immunogen: DIABLO (AAH11909, 119 a.a. ~ 218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4F9

Description: Other names: DIABLOS, FLJ10537, FLJ25049, SMAC, SMAC3 diablo homolog (Drosophila)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DIABLO (AAH11909, 119 a.a. ~ 218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 27131 DA

Comments:

Background: This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of DIABLO expression in transfected 293T cell line by DIABLO monoclonal antibody (M02), clone 4F9. Lane 1: DIABLO transfected lysate(27.131 KDa). Lane 2: Non-transfected lysate. Detection limit for recombinant GST tagged DIABLO is approximately 0.3ng/ml as a capture antibody.

anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody (Image 2)

Publications

Product: Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Dai, Li, Shi et al.: "Survivin and Smac gene expressions but not livin are predictors of prognosis in non-small cell lung cancer patients treated with adjuvant chemotherapy following surgery." in: Japanese journal of clinical oncology, Vol. 40, Issue 4, pp. 327-35, 2010 (PubMed).

Bae, Kho, Lee et al.: "Hip2 interacts with and destabilizes Smac/DIABLO." in: Biochemical and biophysical research communications, Vol. 397, Issue 4, pp. 718-23, 2010 (PubMed).

Lu, Xu, Fan et al.: "Overexpression of Smac/DIABLO in Hep-2 cell line: possible role in potentiating the sensitivity of chemotherapeutic drugs." in: Tumori, Vol. 96, Issue 2, pp. 310-5, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).