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Amino-terminal Enhancer of Split (AES) (AA 2-111) antibody

Details for Product No. ABIN393219
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Antigen
Synonyms grg, esp1, grg5, tle5, aes-1, aes-2, AES, AES-1, AES-2, ESP1, GRG, GRG5, TLE5, AL024115, Esp1, Grg, Grg-5, Grg5, Tle5, zgc:64216, xgrg-5
Epitope
AA 2-111
(24), (16), (7), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(53), (27), (15), (2), (1), (1), (1)
Host
Mouse
(50), (4), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(52), (27), (22), (8), (5), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393219
450.00 $
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Immunogen AES (NP_001121, 2 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1E1
Isotype IgG1 kappa
Specificity AES (NP_001121, 2 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AES
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 21970 DA
Gene ID 12406
UniProt NP_001121
Research Area Neurology, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Amino-terminal Enhancer of Split (AES) (AA 2-111) antibody AES monoclonal antibody (M02), clone 1E1 Western Blot analysis of AES expression in Hela NE. Detection limit for recombinant GST tagged AES is approximately 1 ng/mL as a capture antibody.
anti-Amino-terminal Enhancer of Split (AES) (AA 2-111) antibody (2) anti-Amino-terminal Enhancer of Split (AES) (AA 2-111) antibody (Image 2)
Background publications Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Zhang, Chen, Jaramillo et al.: "Histone deacetylase-related protein inhibits AES-mediated neuronal cell death by direct interaction." in: Journal of neuroscience research, Vol. 86, Issue 11, pp. 2423-31, 2008 (PubMed).

Arce, Pate, Waterman: "Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression." in: BMC cancer, Vol. 9, pp. 159, 2009 (PubMed).

Zhang, Gao, Wang: "Structural and functional analysis of amino-terminal enhancer of split in androgen-receptor-driven transcription." in: The Biochemical journal, Vol. 427, Issue 3, pp. 499-511, 2010 (PubMed).

Validation Images
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