Amino-terminal Enhancer of Split (AES) antibody

Antigen: Amino-terminal Enhancer of Split (AES)

Synonyms: GRG, ESP1, GRG5, TLE5, AES-1, AES-2, Grg, Grg5, AL024115, MGC124620, AES, MGC64216, zgc:64216, aes, MGC68432, grg, esp1, grg5, tle5, aes-1, aes-2

Clonality: Monoclonal (1E1)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393219

Additional Information

Alternative name: AES

Gene ID: 166

UniProt: NP_001121

Immunogen: AES (NP_001121, 2 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1E1

Description: Other names: AES1, AES2, ESP1, GRG, GRG5, TLE5 amino-terminal enhancer of split

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AES (NP_001121, 2 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 21970 DA

Comments:

Background: The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

AES monoclonal antibody (M02), clone 1E1 Western Blot analysis of AES expression in Hela NE ( Cat # L013V3 ). Detection limit for recombinant GST tagged AES is approximately 1ng/ml as a capture antibody.

anti-Amino-terminal Enhancer of Split (AES) antibody (Image 2)

Publications

Product: Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Zhang, Chen, Jaramillo et al.: "Histone deacetylase-related protein inhibits AES-mediated neuronal cell death by direct interaction." in: Journal of neuroscience research, Vol. 86, Issue 11, pp. 2423-31, 2008 (PubMed).

Arce, Pate, Waterman: "Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression." in: BMC cancer, Vol. 9, pp. 159, 2009 (PubMed).

Zhang, Gao, Wang: "Structural and functional analysis of amino-terminal enhancer of split in androgen-receptor-driven transcription." in: The Biochemical journal, Vol. 427, Issue 3, pp. 499-511, 2010 (PubMed).