S100 Calcium Binding Protein A5 (S100A5) antibody

Details for Product No. ABIN393220
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Synonyms S100D, S100D9, S100A5
(29), (18), (15), (2)
(28), (9)
Clonality (Clone)
Monoclonal ()
(5), (3)
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(35), (28), (15), (9), (8), (6), (3), (2)
Pubmed 5 references available
Catalog no. ABIN393220
Quantity 0.1 mg
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Immunogen S100A5 (NP_002953, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5E1
Isotype IgG2a kappa
Specificity S100A5 (NP_002953, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name S100A5
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 10744 DA
Gene ID 12406
UniProt NP_002953
Research Area Neurology, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-S100 Calcium Binding Protein A5 (S100A5) antibody Immunoperoxidase of monoclonal antibody to S100A5 on formalin-fixed paraffin-embedded human kidney (antibody concentration 1.5 µg/mL).
Background publications Chan, Xia, Dong et al.: "Differential expression of S100 proteins in the developing human hippocampus and temporal cortex." in: Microscopy research and technique, Vol. 60, Issue 6, pp. 600-13, 2003 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1...." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Bertini, Das Gupta, Hu et al.: "Solution structure and dynamics of S100A5 in the apo and Ca2+-bound states." in: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, Vol. 14, Issue 7, pp. 1097-107, 2009 (PubMed).

Stemmler, Nothnagel, Parwez et al.: "Variation in genes of the epidermal differentiation complex in German atopic dermatitis patients." in: International journal of immunogenetics, Vol. 36, Issue 4, pp. 217-22, 2009 (PubMed).

Potkin, Guffanti, Lakatos et al.: "Hippocampal atrophy as a quantitative trait in a genome-wide association study identifying novel susceptibility genes for Alzheimer's disease." in: PLoS ONE, Vol. 4, Issue 8, pp. e6501, 2009 (PubMed).

Hosts (28), (9)
Reactivities (29), (18), (15), (2)
Applications (35), (28), (15), (9), (8), (6), (3), (2)
Conjugates (5), (3)
Epitopes (9), (3), (2), (2), (1), (1), (1)
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