S100 Calcium Binding Protein A5 (S100A5) antibody

Antigen: S100 Calcium Binding Protein A5 (S100A5)

Synonyms: S100D, S100D9, S100A5

Clonality: Monoclonal (5E1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393220

Additional Information

Alternative name: S100A5

Gene ID: 6276

UniProt: NP_002953

Immunogen: S100A5 (NP_002953, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 5E1

Description: Other names: S100D S100 calcium binding protein A5

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: S100A5 (NP_002953, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 10744 DA

Comments:

Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to S100A5 ( Cat# AT3758a ) on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5 ug/ml]

Publications

Product: Chan, Xia, Dong et al.: "Differential expression of S100 proteins in the developing human hippocampus and temporal cortex." in: Microscopy research and technique, Vol. 60, Issue 6, pp. 600-13, 2003 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Bertini, Das Gupta, Hu et al.: "Solution structure and dynamics of S100A5 in the apo and Ca2+-bound states." in: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, Vol. 14, Issue 7, pp. 1097-107, 2009 (PubMed).

Stemmler, Nothnagel, Parwez et al.: "Variation in genes of the epidermal differentiation complex in German atopic dermatitis patients." in: International journal of immunogenetics, Vol. 36, Issue 4, pp. 217-22, 2009 (PubMed).

Potkin, Guffanti, Lakatos et al.: "Hippocampal atrophy as a quantitative trait in a genome-wide association study identifying novel susceptibility genes for Alzheimer's disease." in: PLoS ONE, Vol. 4, Issue 8, pp. e6501, 2009 (PubMed).