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S100 Calcium Binding Protein A5 (S100A5) antibody
|Synonyms||S100D, S100D9, S100A5|
Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||S100A5 (NP_002953, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: S100D S100 calcium binding protein A5|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||S100A5 (NP_002953, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||10744 DA|
Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Neurology, Cell Structure|
|Restrictions||For Research Use only|
|Immunoperoxidase of monoclonal antibody to S100A5 ( Cat# AT3758a ) on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5 ug/ml]|
Chan, Xia, Dong et al.: "Differential expression of S100 proteins in the developing human hippocampus and temporal cortex." in: Microscopy research and technique, Vol. 60, Issue 6, pp. 600-13, 2003 (PubMed).
Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).
Bertini, Das Gupta, Hu et al.: "Solution structure and dynamics of S100A5 in the apo and Ca2+-bound states." in: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, Vol. 14, Issue 7, pp. 1097-107, 2009 (PubMed).
Stemmler, Nothnagel, Parwez et al.: "Variation in genes of the epidermal differentiation complex in German atopic dermatitis patients." in: International journal of immunogenetics, Vol. 36, Issue 4, pp. 217-22, 2009 (PubMed).
Potkin, Guffanti, Lakatos et al.: "Hippocampal atrophy as a quantitative trait in a genome-wide association study identifying novel susceptibility genes for Alzheimer's disease." in: PLoS ONE, Vol. 4, Issue 8, pp. e6501, 2009 (PubMed).
|Hosts||Rabbit (19), Mouse (7)|
|Reactivities||Human (20), Mouse (Murine) (11), Rat (Rattus) (11)|
|Applications||Western Blotting (WB) (23), ELISA (19), Immunohistochemistry (IHC) (16), Immunocytochemistry (ICC) (2), Immunofluorescence (IF) (2), Immunostaining (ISt) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)|
|Conjugates||Biotin (3), FITC (2)|
|Epitopes||Internal Region (7), Middle Region (1)|