Hypocretin (Orexin) Receptor 2 (HCRTR2) antibody

Antigen: Hypocretin (Orexin) Receptor 2 (HCRTR2)

Synonyms: OX, PPOX, NRCLP1, MGC151320, MGC151346, orexin-A, HCRTR2, HCRT, LOC100229747, OX2R, LOC443181, hcrtr

Clonality: Monoclonal (1E3)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393222

Additional Information

Alternative name: HCRTR2

Gene ID: 3062

UniProt: NP_001517

Immunogen: HCRTR2 (NP_001517, 1 a.a. ~ 55 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1E3

Description: Other names: OX2R hypocretin (orexin) receptor 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: HCRTR2 (NP_001517, 1 a.a. ~ 55 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 50694 DA

Comments:

Background: The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of HCRTR2 expression in transfected 293T cell line by HCRTR2 monoclonal antibody (M01), clone 1E3. Lane 1: HCRTR2 transfected lysate(50.7 KDa). Lane 2: Non-transfected lysate. Western blot analysis of HCRTR2 over-expressed 293 cell line, cotransfected with HCRTR2 Validated Chimera RNAi ( Cat # H00003062-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HCRTR2 monoclonal antibody (M01), clone 1E3 (Cat # AT2331a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Hypocretin (Orexin) Receptor 2 (HCRTR2) antibody (Image 2)

Publications

Product: Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Fellay, Ge, Shianna et al.: "Common genetic variation and the control of HIV-1 in humans." in: PLoS genetics, Vol. 5, Issue 12, pp. e1000791, 2009 (PubMed).

Chen, Randeva: "Genomic organization and regulation of the human orexin (hypocretin) receptor 2 gene: identification of alternative promoters." in: The Biochemical journal, Vol. 427, Issue 3, pp. 377-90, 2010 (PubMed).

Pinheiro, Bulik, Thornton et al.: "Association study of 182 candidate genes in anorexia nervosa." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 153B, Issue 5, pp. 1070-80, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).