Neurofilament, Heavy Polypeptide (NEFH) antibody

Antigen: Neurofilament, Heavy Polypeptide (NEFH)

Clonality: Monoclonal (2E8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393231

Additional Information

Alternative name: NEFH

Gene ID: 4744

UniProt: NP_066554

Immunogen: NEFH (NP_066554, 263 a.a. ~ 364 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2E8

Description: Other names: NFH neurofilament, heavy polypeptide 200kDa

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: NEFH (NP_066554, 263 a.a. ~ 364 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 112480 DA

Comments:

Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

NEFH monoclonal antibody (M01), clone 2E8 Western Blot analysis of NEFH expression in HeLa ( Cat # L013V1 ). Detection limit for recombinant GST tagged NEFH is approximately 0.03ng/ml as a capture antibody.

anti-Neurofilament, Heavy Polypeptide (NEFH) antibody (Image 2)

Publications

Product: Rudrabhatla, Zheng, Amin et al.: "Pin1-dependent prolyl isomerization modulates the stress-induced phosphorylation of high molecular weight neurofilament protein." in: The Journal of biological chemistry, Vol. 283, Issue 39, pp. 26737-47, 2008 (PubMed).

Gregório, Sallet, Do et al.: "Polymorphisms in genes involved in neurodevelopment may be associated with altered brain morphology in schizophrenia: preliminary evidence." in: Psychiatry research, Vol. 165, Issue 1-2, pp. 1-9, 2008 (PubMed).

Guy, Shaw, Ross-Cisneros et al.: "Phosphorylated neurofilament heavy chain is a marker of neurodegeneration in Leber hereditary optic neuropathy (LHON)." in: Molecular vision, Vol. 14, pp. 2443-50, 2008 (PubMed).

Alberti, Gonzalez, Maldonado et al.: "Comparative study of CSF neurofilaments in HTLV-1-associated myelopathy/tropical spastic paraparesis and other neurological disorders." in: AIDS research and human retroviruses, Vol. 25, Issue 8, pp. 803-9, 2009 (PubMed).

Shrestha, Irvin, Taylor et al.: "A genome-wide association study of carotid atherosclerosis in HIV-infected men." in: AIDS (London, England), Vol. 24, Issue 4, pp. 583-92, 2010 (PubMed).