Neurofilament, Heavy Polypeptide (NEFH) (AA 263-364) antibody

Details for Product No. ABIN393231, Supplier: Log in to see
Antigen
  • NF-H
  • NF200
  • Nfh
  • mKIAA0845
  • NFH
  • NEFH
  • neurofilament heavy polypeptide
  • neurofilament, heavy polypeptide
  • MCYG_06231
  • Nefh
  • NEFH
Epitope
AA 263-364
32
19
15
8
4
3
2
1
1
1
1
1
1
1
1
Reactivity
Human
384
340
321
240
198
97
80
79
56
54
54
52
31
27
26
25
17
3
2
2
2
1
Host
Mouse
349
53
17
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
13
12
12
11
11
11
11
11
11
11
11
11
11
11
9
9
8
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
232
209
146
134
118
108
68
55
13
12
9
2
2
2
1
Options
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Immunogen NEFH (NP_066554, 263 a.a. ~ 364 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2E8
Isotype IgG2a kappa
Specificity NEFH (NP_066554, 263 a.a. ~ 364 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NEFH (NEFH Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 112480 DA
Gene ID 12406
NCBI Accession NP_066554
Research Area Neurology, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 263-364) antibody (ABIN393231) NEFH monoclonal antibody (M01), clone 2E8 Western Blot analysis of NEFH expression in...
Immunohistochemistry (IHC) image for anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 263-364) antibody (ABIN393231) anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 263-364) antibody (Image 2)
Background publications Shrestha, Irvin, Taylor, Wiener, Pajewski, Haritunians, Delaney, Schambelan, Polak, Arnett, Chen, Grunfeld: "A genome-wide association study of carotid atherosclerosis in HIV-infected men." in: AIDS, Vol. 24, Issue 4, pp. 583-92, 2010 (PubMed).

Alberti, Gonzalez, Maldonado, Medina, Barriga, García, Kettlun, Collados, Puente, Cartier, Valenzuela: "Comparative study of CSF neurofilaments in HTLV-1-associated myelopathy/tropical spastic paraparesis and other neurological disorders." in: AIDS research and human retroviruses, Vol. 25, Issue 8, pp. 803-9, 2009 (PubMed).

Guy, Shaw, Ross-Cisneros, Quiros, Salomao, Berezovsky, Carelli, Feuer, Sadun: "Phosphorylated neurofilament heavy chain is a marker of neurodegeneration in Leber hereditary optic neuropathy (LHON)." in: Molecular vision, Vol. 14, pp. 2443-50, 2008 (PubMed).

Gregório, Sallet, Do, Lin, Gattaz, Dias-Neto: "Polymorphisms in genes involved in neurodevelopment may be associated with altered brain morphology in schizophrenia: preliminary evidence." in: Psychiatry research, Vol. 165, Issue 1-2, pp. 1-9, 2008 (PubMed).

Rudrabhatla, Zheng, Amin, Kesavapany, Albers, Pant: "Pin1-dependent prolyl isomerization modulates the stress-induced phosphorylation of high molecular weight neurofilament protein." in: The Journal of biological chemistry, Vol. 283, Issue 39, pp. 26737-47, 2008 (PubMed).