Sonic Hedgehog (SHH) antibody

Antigen: Sonic Hedgehog (SHH)

Synonyms: TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5, Hx, Dsh, Hhg1, Hxl3, M100081, 9530036O11Rik, shh, syu, vhh1, wu:fc83d08, SHH

Clonality: Monoclonal (1B11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN393243

Additional Information

Alternative name: SHH

Gene ID: 6469

UniProt: NP_000184

Immunogen: SHH (NP_000184, 181 a.a. ~ 281 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1B11

Description: Other names: HHG1, HLP3, HPE3, SMMCI sonic hedgehog homolog (Drosophila)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: SHH (NP_000184, 181 a.a. ~ 281 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 49607 DA

Comments:

Background: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged SHH is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Hatton, Villavicencio, Pritchard et al.: "Notch signaling is not essential in sonic hedgehog-activated medulloblastoma." in: Oncogene, Vol. 29, Issue 26, pp. 3865-72, 2010 (PubMed).

Lauth, Bergström, Shimokawa et al.: "DYRK1B-dependent autocrine-to-paracrine shift of Hedgehog signaling by mutant RAS." in: Nature structural & molecular biology, Vol. 17, Issue 6, pp. 718-25, 2010 (PubMed).

Johnson, Yanek, Chen et al.: "Genome-wide meta-analyses identifies seven loci associated with platelet aggregation in response to agonists." in: Nature genetics, Vol. 42, Issue 7, pp. 608-13, 2010 (PubMed).

Choi, Witek, Yang et al.: "Activation of Rac1 promotes hedgehog-mediated acquisition of the myofibroblastic phenotype in rat and human hepatic stellate cells." in: Hepatology (Baltimore, Md.), Vol. 52, Issue 1, pp. 278-90, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).