Protocadherin gamma Subfamily A, 8 (PCDHGA8) (AA 357-445) antibody

Details for Product No. ABIN393250
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Antigen
Synonyms PCDH-GAMMA-A8, PCDHGB5, mKIAA4054
Epitope
AA 357-445
(9), (8), (2), (2), (2)
Reactivity
Human
(27), (13), (13), (13)
Host
Mouse
(23), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(17), (11), (10), (8), (8), (6), (5), (4), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393250
450.00 $
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Immunogen PCDHGA8 (NP_114477, 357 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1C11
Isotype IgG2a kappa
Specificity PCDHGA8 (NP_114477, 357 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PCDHGA8
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 101480 DA
Gene ID 12406
NCBI Accession NP_114477
Research Area Cell Structure, Neurology
Application Notes Western blot = 1:500-1000
Comment

Background: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Protocadherin gamma Subfamily A, 8 (PCDHGA8) (AA 357-445) antibody Western Blot analysis of PCDHGA8 expression in transfected 293T cell line by PCDHGA8 monoclonal antibody (M01), clone 1C11. Lane 1: PCDHGA8 transfected lysate(101.48 KDa). Lane 2: Non-transfected lysate. Western blot analysis of PCDHGA8 over-expressed 293 cell line, cotransfected with PCDHGA8 Validated Chimera RNAi used as specificity and loading control.
anti-Protocadherin gamma Subfamily A, 8 (PCDHGA8) (AA 357-445) antibody (2) anti-Protocadherin gamma Subfamily A, 8 (PCDHGA8) (AA 357-445) antibody (Image 2)
Background publications Wu, Maniatis: "A striking organization of a large family of human neural cadherin-like cell adhesion genes." in: Cell, Vol. 97, Issue 6, pp. 779-90, 1999 (PubMed).

Wu, Maniatis: "Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3124-9, 2000 (PubMed).

Yagi, Takeichi: "Cadherin superfamily genes: functions, genomic organization, and neurologic diversity." in: Genes & development, Vol. 14, Issue 10, pp. 1169-80, 2000 (PubMed).

Nollet, Kools, van Roy: "Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members." in: Journal of molecular biology, Vol. 299, Issue 3, pp. 551-72, 2000 (PubMed).

Wu, Zhang, Cheng et al.: "Comparative DNA sequence analysis of mouse and human protocadherin gene clusters." in: Genome research, Vol. 11, Issue 3, pp. 389-404, 2001 (PubMed).

Validation Images
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