Crystallin, mu (CRYM) antibody

Antigen: Crystallin, mu (CRYM)

Synonyms: THBP, DFNA40, MGC108623, CRYM, MGC137343, zgc:158843

Clonality: Monoclonal (1C6)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393252

Additional Information

Alternative name: CRYM

Gene ID: 1428

UniProt: NP_001879

Immunogen: CRYM (NP_001879, 215 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1C6

Description: Other names: DFNA40, THBP crystallin, mu

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CRYM (NP_001879, 215 a.a. ~ 315 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 33776 DA

Comments:

Background: Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. Multiple alternatively spliced transcript variants have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

CRYM monoclonal antibody (M09), clone 1C6. Western Blot analysis of CRYM expression in K-562 ( Cat # L009V1 ). Detection limit for recombinant GST tagged CRYM is approximately 0.03ng/ml as a capture antibody.

anti-Crystallin, mu (CRYM) antibody (Image 2)

Publications

Product: Martins-de-Souza, Gattaz, Schmitt et al.: "Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia." in: Journal of psychiatric research, Vol. 43, Issue 11, pp. 978-86, 2009 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia." in: European archives of psychiatry and clinical neuroscience, Vol. 259, Issue 3, pp. 151-63, 2009 (PubMed).

Malinowska, Cavarretta, Susani et al.: "Identification of mu-crystallin as an androgen-regulated gene in human prostate cancer." in: The Prostate, Vol. 69, Issue 10, pp. 1109-18, 2009 (PubMed).

Al-Kafaji, Malik: "Hyperglycemia induces elevated expression of thyroid hormone binding protein in vivo in kidney and heart and in vitro in mesangial cells." in: Biochemical and biophysical research communications, Vol. 391, Issue 4, pp. 1585-91, 2010 (PubMed).

Martins-de-Souza, Maccarrone, Wobrock et al.: "Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia." in: Journal of psychiatric research, Vol. 44, Issue 16, pp. 1176-89, 2010 (PubMed).