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PCDHA12 antibody (Protocadherin alpha 12) (AA 222-328)

Details for Product anti-PCDHA12 Antibody No. ABIN393255, Supplier: Log in to see
Antigen
  • CNRv12
  • PCDHA12
  • PCDH-ALPHA12
  • Cnr5
  • Crnr5
  • Pcdha13
  • Pcdha11
  • rCNRv12
  • protocadherin alpha 12
  • PCDHA12
  • Pcdha12
Epitope
AA 222-328
15
9
8
2
2
1
1
Reactivity
Human
48
7
5
2
2
1
1
1
1
Host
Mouse
45
3
Clonality (Clone)
Monoclonal ()
Conjugate
This PCDHA12 antibody is un-conjugated
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA
30
18
16
14
13
6
2
2
Options
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Immunogen PCDHA12 (NP_061726, 222 a.a. ~ 328 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D3
Isotype IgG2b kappa
Specificity PCDHA12 (NP_061726, 222 a.a. ~ 328 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PCDHA12 (PCDHA12 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 101652 DA
Gene ID 12406
NCBI Accession NP_061726
Research Area Cell Structure, Neurology
Application Notes ELISA ~~ 1ug/ml~3ng/ml
Comment

Background: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-PCDHA12 antibody (Protocadherin alpha 12) (AA 222-328) (ABIN393255) Detection limit for recombinant GST tagged PCDHA12 is approximately 3 ng/mL as a capt...
Background publications Wu, Ma, Brown, Geisler, Li, Tzeng, Jia, Jurisica, Li: "Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening." in: Proteomics, Vol. 7, Issue 11, pp. 1775-85, 2007 (PubMed).

Otsuki, Ota, Nishikawa, Hayashi, Suzuki, Yamamoto, Wakamatsu, Kimura, Sakamoto, Hatano, Kawai, Ishii, Saito, Kojima, Sugiyama, Ono, Okano, Yoshikawa, Aotsuka, Sasaki, Hattori, Okumura, Nagai, Sugano et al.: "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. ..." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 12, Issue 2, pp. 117-26, 2005 (PubMed).

Schmutz, Martin, Terry, Couronne, Grimwood, Lowry, Gordon, Scott, Xie, Huang, Hellsten, Tran-Gyamfi, She, Prabhakar, Aerts, Altherr, Bajorek, Black, Branscomb, Caoile, Challacombe, Chan, Denys et al.: "The DNA sequence and comparative analysis of human chromosome 5. ..." in: Nature, Vol. 431, Issue 7006, pp. 268-74, 2004 (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Wu, Zhang, Cheng, Kim, Grimwood, Schmutz, Dickson, Noonan, Zhang, Myers, Maniatis: "Comparative DNA sequence analysis of mouse and human protocadherin gene clusters." in: Genome research, Vol. 11, Issue 3, pp. 389-404, 2001 (PubMed).