Distal-Less Homeobox 4 (DLX4) antibody

Antigen: Distal-Less Homeobox 4 (DLX4)

Synonyms: BP1, DLX7, DLX8, DLX9, Dlx7, Dlx-4, MGC129167, DLX4, MGC149037

Clonality: Monoclonal (1F11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393260

Additional Information

Alternative name: DLX4

Gene ID: 1748

Immunogen: DLX4 (AAH16145, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1F11

Description: Other names: BP1, DLX7, DLX8, DLX9 distal-less homeobox 4

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DLX4 (AAH16145, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 26263 DA

Comments:

Background: Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. Three transcript variants have been described for this gene, however, the full length nature of one variant has not been described. Studies of the two splice variants revealed that one encoded isoform functions as a repressor of the beta-globin gene while the other isoform lacks that function.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of DLX4 over-expressed 293 cell line, cotransfected with DLX4 Validated Chimera RNAi ( Cat # H00001748-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DLX4 monoclonal antibody (M01), clone 1F11 (Cat # AT1780a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of DLX4 expression in transfected 293T cell line by DLX4 monoclonal antibody (M01), clone 1F11. Lane 1: DLX4 transfected lysate(26 KDa). Lane 2: Non-transfected lysate.

anti-Distal-Less Homeobox 4 (DLX4) antibody (Image 2)

Publications

Product: Schwartz, Man, Rezaei et al.: "BP1, a homeoprotein, is significantly expressed in prostate adenocarcinoma and is concordant with prostatic intraepithelial neoplasia." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 1, pp. 1-6, 2008 (PubMed).

Vieira, McHenry, Daack-Hirsch et al.: "Candidate gene/loci studies in cleft lip/palate and dental anomalies finds novel susceptibility genes for clefts." in: Genetics in medicine : official journal of the American College of Medical Genetics, Vol. 10, Issue 9, pp. 668-74, 2008 (PubMed).

Cavalli, Man, Schwartz et al.: "Amplification of the BP1 homeobox gene in breast cancer." in: Cancer genetics and cytogenetics, Vol. 187, Issue 1, pp. 19-24, 2008 (PubMed).

Man, Schwartz, Levine et al.: "BP1, a putative signature marker for inflammatory breast cancer and tumor aggressiveness." in: Cancer biomarkers : section A of Disease markers, Vol. 5, Issue 1, pp. 9-17, 2009 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).