Huntingtin Interacting Protein 1 (HIP1) (AA 928-1038) antibody

Details for Product No. ABIN393261
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Antigen
Synonyms CG10971, Dmel\\CG10971, Hip1R, hip1, MGC78813, HIP-I, ILWEQ, 2610109B09Rik, A930014B11Rik, E130315I21Rik, mKIAA4113
Epitope
AA 928-1038
(7), (4), (3), (3), (1)
Reactivity
Human
(53), (17), (15), (12), (12)
Host
Mouse
(32), (23)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(43), (19), (11), (10), (9), (4), (3), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393261
450.00 $
Plus shipping costs $45.00

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Immunogen HIP1 (NP_005329, 928 a.a. ~ 1038 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1F12
Isotype IgG2a kappa
Specificity HIP1 (NP_005329, 928 a.a. ~ 1038 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name HIP1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 116221 DA
Gene ID 12406
NCBI Accession NP_005329
Research Area Cell Structure, Neurology
Application Notes Western blot = 1:500-1000
Comment

Background: The product of this gene is a membrane-associated protein that colocalizes with huntingtin. This protein has similarities to cytoskeleton proteins and its interaction with huntingtin is thought to play a functional role in the cell filament network. Loss of normal huntingtin-HIP1 interaction in Huntington disease may contribute to a defect in membrane-cytoskeletal integrity in the brain. This gene could help in the understanding of the normal function of huntingtin and also the pathogenesis of Huntington disease. It also has been implicated in the pathogenesis of hematopoietic malignancies. An alternative splice variant of this gene has been described but its full length sequence has not been determined.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Huntingtin Interacting Protein 1 (HIP1) (AA 928-1038) antibody HIP1 monoclonal antibody (M01), clone 1F12 Western Blot analysis of HIP1 expression in HeLa on formalin-fixed paraffin-embedded human colon (antibody concentration 3 µg/mL).
anti-Huntingtin Interacting Protein 1 (HIP1) (AA 928-1038) antibody (2) anti-Huntingtin Interacting Protein 1 (HIP1) (AA 928-1038) antibody (Image 2)
Background publications Niu, Ybe: "Crystal structure at 2.8 A of Huntingtin-interacting protein 1 (HIP1) coiled-coil domain reveals a charged surface suitable for HIP1 protein interactor (HIPPI)." in: Journal of molecular biology, Vol. 375, Issue 5, pp. 1197-205, 2008 (PubMed).

Wilbur, Chen, Manalo et al.: "Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain." in: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32870-9, 2008 (PubMed).

Gottfried, Ehrlich, Ashery: "HIP1 exhibits an early recruitment and a late stage function in the maturation of coated pits." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 17, pp. 2897-911, 2009 (PubMed).

Han, Zheng, Cui et al.: "Genome-wide association study in a Chinese Han population identifies nine new susceptibility loci for systemic lupus erythematosus...." in: Nature genetics, Vol. 41, Issue 11, pp. 1234-7, 2009 (PubMed).

Wilbur, Hwang, Brodsky et al.: "Accommodation of structural rearrangements in the huntingtin-interacting protein 1 coiled-coil domain." in: Acta crystallographica. Section D, Biological crystallography, Vol. 66, Issue Pt 3, pp. 314-8, 2010 (PubMed).

Validation Images
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