Huntingtin Interacting Protein 1 (HIP1) antibody

Antigen: Huntingtin Interacting Protein 1 (HIP1)

Synonyms: ILWEQ, MGC126506, CG10971, DmelCG10971, KIAA4113, MGC27616, mKIAA4113, 2610109B09Rik, A930014B11Rik, E130315I21Rik, HYPB, SET2, HIF-1, KMT3A, HBP231, HSPC069, p231HBP, FLJ16420, FLJ22472, FLJ23184, FLJ45883, FLJ46217, KIAA1732, hip1, MGC78813, HIP1

Clonality: Monoclonal (1F12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393261

Additional Information

Alternative name: HIP1

Gene ID: 3092

UniProt: NP_005329

Immunogen: HIP1 (NP_005329, 928 a.a. ~ 1038 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1F12

Description: Other names: huntingtin interacting protein 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: HIP1 (NP_005329, 928 a.a. ~ 1038 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 116221 DA

Comments:

Background: The product of this gene is a membrane-associated protein that colocalizes with huntingtin. This protein has similarities to cytoskeleton proteins and its interaction with huntingtin is thought to play a functional role in the cell filament network. Loss of normal huntingtin-HIP1 interaction in Huntington disease may contribute to a defect in membrane-cytoskeletal integrity in the brain. This gene could help in the understanding of the normal function of huntingtin and also the pathogenesis of Huntington disease. It also has been implicated in the pathogenesis of hematopoietic malignancies. An alternative splice variant of this gene has been described but its full length sequence has not been determined.

Synonyms: ILWEQ, MGC126506, CG10971, DmelCG10971, KIAA4113, MGC27616, mKIAA4113, 2610109B09Rik, A930014B11Rik, E130315I21Rik, HYPB, SET2, HIF-1, KMT3A, HBP231, HSPC069, p231HBP, FLJ16420, FLJ22472, FLJ23184, FLJ45883, FLJ46217, KIAA1732, hip1, MGC78813, HIP1

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Neurology

Restrictions: For Research Use only

Images

HIP1 monoclonal antibody (M01), clone 1F12 Western Blot analysis of HIP1 expression in HeLa ( Cat # L013V1 ). Immunoperoxidase of monoclonal antibody to HIP1 ( Cat # AT2370a ) on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml]

anti-Huntingtin Interacting Protein 1 (HIP1) antibody (Image 2)

Publications

Product: Niu, Ybe: "Crystal structure at 2.8 A of Huntingtin-interacting protein 1 (HIP1) coiled-coil domain reveals a charged surface suitable for HIP1 protein interactor (HIPPI)." in: Journal of molecular biology, Vol. 375, Issue 5, pp. 1197-205, 2008 (PubMed).

Wilbur, Chen, Manalo et al.: "Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain." in: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32870-9, 2008 (PubMed).

Gottfried, Ehrlich, Ashery: "HIP1 exhibits an early recruitment and a late stage function in the maturation of coated pits." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 17, pp. 2897-911, 2009 (PubMed).

Han, Zheng, Cui et al.: "Genome-wide association study in a Chinese Han population identifies nine new susceptibility loci for systemic lupus erythematosus." in: Nature genetics, Vol. 41, Issue 11, pp. 1234-7, 2009 (PubMed).

Wilbur, Hwang, Brodsky et al.: "Accommodation of structural rearrangements in the huntingtin-interacting protein 1 coiled-coil domain." in: Acta crystallographica. Section D, Biological crystallography, Vol. 66, Issue Pt 3, pp. 314-8, 2010 (PubMed).