Protocadherin alpha 10 (PCDHA10) antibody

Antigen: Protocadherin alpha 10 (PCDHA10)

Synonyms: CNR8, CNRN8, CNRS8, CRNR8, PCDH-ALPHA10

Clonality: Monoclonal (1F6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393264

Additional Information

Alternative name: PCDHA10

Gene ID: 56139

UniProt: NP_061724

Immunogen: PCDHA10 (NP_061724, 182 a.a. ~ 291 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1F6

Description: Other names: CNR8, CNRN8, CNRS8, CRNR8, PCDHALPHA10 protocadherin alpha 10

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: PCDHA10 (NP_061724, 182 a.a. ~ 291 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 102875 DA

Comments:

Background: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Neurology

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to PCDHA10 ( Cat# AT3217a ) on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]

Publications

Product: Nollet, Kools, van Roy: "Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members." in: Journal of molecular biology, Vol. 299, Issue 3, pp. 551-72, 2000 (PubMed).

Wu, Zhang, Cheng et al.: "Comparative DNA sequence analysis of mouse and human protocadherin gene clusters." in: Genome research, Vol. 11, Issue 3, pp. 389-404, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Schmutz, Martin, Terry et al.: "The DNA sequence and comparative analysis of human chromosome 5." in: Nature, Vol. 431, Issue 7006, pp. 268-74, 2004 (PubMed).

Wu, Ma, Brown et al.: "Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening." in: Proteomics, Vol. 7, Issue 11, pp. 1775-85, 2007 (PubMed).