Protocadherin gamma Subfamily A, 2 (PCDHGA2) antibody

Antigen: Protocadherin gamma Subfamily A, 2 (PCDHGA2)

Synonyms: PCDH-GAMMA-A2

Clonality: Monoclonal (2A7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393281

Additional Information

Alternative name: PCDHGA2

Gene ID: 56113

UniProt: NP_061738

Immunogen: PCDHGA2 (NP_061738, 223 a.a. ~ 332 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2A7

Description: Other names: PCDHGAMMAA2 protocadherin gamma subfamily A, 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: PCDHGA2 (NP_061738, 223 a.a. ~ 332 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 101484 DA

Comments:

Background: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of PCDHGA2 over-expressed 293 cell line, cotransfected with PCDHGA2 Validated Chimera RNAi ( Cat # H00056113-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDHGA2 monoclonal antibody (M01), clone 2A7 (Cat # AT3227a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of PCDHGA2 expression in transfected 293T cell line by PCDHGA2 monoclonal antibody (M01), clone 2A7. Lane 1: PCDHGA2 transfected lysate(90.3 KDa). Lane 2: Non-transfected lysate.

anti-Protocadherin gamma Subfamily A, 2 (PCDHGA2) antibody (Image 2)

Publications

Product: Yagi, Takeichi: "Cadherin superfamily genes: functions, genomic organization, and neurologic diversity." in: Genes & development, Vol. 14, Issue 10, pp. 1169-80, 2000 (PubMed).

Nollet, Kools, van Roy: "Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members." in: Journal of molecular biology, Vol. 299, Issue 3, pp. 551-72, 2000 (PubMed).

Wu, Zhang, Cheng et al.: "Comparative DNA sequence analysis of mouse and human protocadherin gene clusters." in: Genome research, Vol. 11, Issue 3, pp. 389-404, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).