Glutamate-Ammonia Ligase (GLUL) antibody

Antigen: Glutamate-Ammonia Ligase (GLUL)

Synonyms: glul, MGC64262, GS, GLNS, PIG43, PIG59, Glns, GLUL, MGC128403, xgs, F15E12.14, F15E12_14, GLUTAMINE SYNTHETASE

Clonality: Monoclonal (2B12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393284

Additional Information

Alternative name: GLUL

Gene ID: 2752

Immunogen: GLUL (AAH10037, 1 a.a. ~ 374 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2B12

Description: Other names: GLNS, GS glutamate-ammonia ligase (glutamine synthetase)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: GLUL (AAH10037, 1 a.a. ~ 374 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 42064 DA

Comments:

Background: The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. This gene is expressed during early fetal stages, and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in this gene are associated with congenital glutamine deficiency. Several alternatively spliced transcript variants have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Signaling, Cell Cycle, Neurology, Cell Structure

Restrictions: For Research Use only

Images

GLUL monoclonal antibody (M01), clone 2B12 Western Blot analysis of GLUL expression in HeLa ( Cat # L013V1 ). Immunoperoxidase of monoclonal antibody to GLUL ( Cat # AT2214a ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.5 ug/ml]

anti-Glutamate-Ammonia Ligase (GLUL) antibody (Image 2)

Publications

Product: Di Tommaso, Destro, Seok et al.: "The application of markers (HSP70 GPC3 and GS) in liver biopsies is useful for detection of hepatocellular carcinoma." in: Journal of hepatology, Vol. 50, Issue 4, pp. 746-54, 2009 (PubMed).

Xin, Rual, Hirozane-Kishikawa et al.: "Shifted Transversal Design smart-pooling for high coverage interactome mapping." in: Genome research, Vol. 19, Issue 7, pp. 1262-9, 2009 (PubMed).

Jozwik, Pietrzycki, Jozwik et al.: "Expression of enzymes regulating placental ammonia homeostasis in human fetal growth restricted pregnancies." in: Placenta, Vol. 30, Issue 7, pp. 607-12, 2009 (PubMed).

Dal Bello, Rosa, Campanini et al.: "Glutamine synthetase immunostaining correlates with pathologic features of hepatocellular carcinoma and better survival after radiofrequency thermal ablation." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 7, pp. 2157-66, 2010 (PubMed).

Martins-de-Souza, Maccarrone, Wobrock et al.: "Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia." in: Journal of psychiatric research, Vol. 44, Issue 16, pp. 1176-89, 2010 (PubMed).