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PCDH20 antibody (Protocadherin 20) (AA 395-495)

Details for Product anti-PCDH20 Antibody No. ABIN393292, Supplier: Log in to see
Antigen
  • C630015B17Rik
  • Pcdh13
  • PCDH13
  • pcdh13
  • PCDH20
Epitope
AA 395-495
16
11
7
3
3
2
1
1
1
Reactivity
Human
41
13
13
Host
Mouse
36
5
Clonality (Clone)
Monoclonal ()
Conjugate
This PCDH20 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
27
19
17
15
11
11
7
6
1
Supplier
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Immunogen PCDH20 (NP_073754, 395 a.a. ~ 495 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C3
Isotype IgG2a kappa
Specificity PCDH20 (NP_073754, 395 a.a. ~ 495 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PCDH20 (PCDH20 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 101927 DA
Gene ID 12406
NCBI Accession NP_073754
Research Area Cell Structure, Neurology
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-PCDH20 antibody (Protocadherin 20) (AA 395-495) (ABIN393292) Detection limit for recombinant GST tagged PCDH20 is approximately 1 ng/mL as a captu...
Background publications Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Suzuki: "Recent progress in protocadherin research." in: Experimental cell research, Vol. 261, Issue 1, pp. 13-8, 2000 (PubMed).

Nollet, Kools, van Roy: "Phylogenetic analysis of the cadherin superfamily allows identification of six major subfamilies besides several solitary members." in: Journal of molecular biology, Vol. 299, Issue 3, pp. 551-72, 2000 (PubMed).