|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Aldolase B, Fructose-Bisphosphate (ALDOB) antibody
|Synonyms||ALDB, ALDO2, Aldo2, Aldo-2, BC016435, MGC36398, fb25f09, wu:fb25f09, MGC53389, MGC76327, MGC86365, ALDOB, MGC127229, aldob, DKFZp469P0832|
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||ALDOB (AAH29399, 1 a.a. ~ 317 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: aldolase B, fructose-bisphosphate|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||ALDOB (AAH29399, 1 a.a. ~ 317 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||39473 DA|
Background: Fructose-1,6-bisphosphate aldolase (EC 22.214.171.124) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Neurology, Cell Structure|
|Restrictions||For Research Use only|
|Detection limit for recombinant GST tagged ALDOB is approximately 0.3ng/ml as a capture antibody.|
Davit-Spraul, Costa, Zater et al.: "Hereditary fructose intolerance: frequency and spectrum mutations of the aldolase B gene in a large patients cohort from France--identification of eight new mutations." in: Molecular genetics and metabolism, Vol. 94, Issue 4, pp. 443-7, 2008 (PubMed).
Eriksson, Flach, Lindgren et al.: "Five mucosal transcripts of interest in ulcerative colitis identified by quantitative real-time PCR: a prospective study." in: BMC gastroenterology, Vol. 8, pp. 34, 2008 (PubMed).
Segat, Milanese, Pirulli et al.: "Secreted protein acidic and rich in cysteine (SPARC) gene polymorphism association with hepatocellular carcinoma in Italian patients." in: Journal of gastroenterology and hepatology, Vol. 24, Issue 12, pp. 1840-6, 2009 (PubMed).
Coffee, Yerkes, Ewen et al.: "Increased prevalence of mutant null alleles that cause hereditary fructose intolerance in the American population." in: Journal of inherited metabolic disease, Vol. 33, Issue 1, pp. 33-42, 2010 (PubMed).
Bouteldja, Timson: "The biochemical basis of hereditary fructose intolerance." in: Journal of inherited metabolic disease, Vol. 33, Issue 2, pp. 105-12, 2010 (PubMed).
|Hosts||Rabbit (33), Mouse (7)|
|Reactivities||Human (38), Rat (Rattus) (29), Mouse (Murine) (26), Cow (Bovine) (21), Pig (Porcine) (19), Sheep (Ovine) (19), Cat (Feline) (1), Chicken (1), Dog (Canine) (1), Rabbit (1)|
|Applications||Western Blotting (WB) (24), Flow Cytometry (FACS) (18), ELISA (15), Immunohistochemistry (IHC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunofluorescence (IF) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Internal Region (2), pSer14 (1)|