Aldolase B, Fructose-Bisphosphate (ALDOB) antibody

Antigen: Aldolase B, Fructose-Bisphosphate (ALDOB)

Synonyms: ALDB, ALDO2, Aldo2, Aldo-2, BC016435, MGC36398, fb25f09, wu:fb25f09, MGC53389, MGC76327, MGC86365, ALDOB, MGC127229, aldob, DKFZp469P0832

Clonality: Monoclonal (3B3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN393315

Additional Information

Alternative name: ALDOB

Gene ID: 229

Immunogen: ALDOB (AAH29399, 1 a.a. ~ 317 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3B3

Description: Other names: aldolase B, fructose-bisphosphate

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ALDOB (AAH29399, 1 a.a. ~ 317 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 39473 DA

Comments:

Background: Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged ALDOB is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Davit-Spraul, Costa, Zater et al.: "Hereditary fructose intolerance: frequency and spectrum mutations of the aldolase B gene in a large patients cohort from France--identification of eight new mutations." in: Molecular genetics and metabolism, Vol. 94, Issue 4, pp. 443-7, 2008 (PubMed).

Eriksson, Flach, Lindgren et al.: "Five mucosal transcripts of interest in ulcerative colitis identified by quantitative real-time PCR: a prospective study." in: BMC gastroenterology, Vol. 8, pp. 34, 2008 (PubMed).

Segat, Milanese, Pirulli et al.: "Secreted protein acidic and rich in cysteine (SPARC) gene polymorphism association with hepatocellular carcinoma in Italian patients." in: Journal of gastroenterology and hepatology, Vol. 24, Issue 12, pp. 1840-6, 2009 (PubMed).

Coffee, Yerkes, Ewen et al.: "Increased prevalence of mutant null alleles that cause hereditary fructose intolerance in the American population." in: Journal of inherited metabolic disease, Vol. 33, Issue 1, pp. 33-42, 2010 (PubMed).

Bouteldja, Timson: "The biochemical basis of hereditary fructose intolerance." in: Journal of inherited metabolic disease, Vol. 33, Issue 2, pp. 105-12, 2010 (PubMed).