Aldolase B, Fructose-Bisphosphate (ALDOB) (AA 1-317) antibody

Details for Product No. ABIN393315, Supplier: Log in to see
Antigen
  • aldb
  • aldo2
  • xaldb
  • ALDOB
  • aldob
  • ALDB
  • ALDO2
  • Aldo-2
  • Aldo2
  • BC016435
  • LIV10
  • fb25f09
  • wu:fb25f09
  • aldolase B
  • aldolase B, fructose-bisphosphate
  • aldolase b, fructose-bisphosphate
  • xaldb
  • aldob
  • ALDOB
  • Aldob
Epitope
AA 1-317
11
9
8
5
5
4
4
3
3
2
2
1
1
1
1
Reactivity
Human
90
34
34
7
5
4
4
4
4
3
3
1
1
Host
Mouse
61
30
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
ELISA
85
45
24
18
10
9
7
4
3
1
1
Options
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Immunogen ALDOB (AAH29399, 1 a.a. ~ 317 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3B3
Isotype IgG2a kappa
Specificity ALDOB (AAH29399, 1 a.a. ~ 317 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ALDOB (ALDOB Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 39473 DA
Gene ID 12406
Research Area Neurology, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml
Comment

Background: Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-Aldolase B, Fructose-Bisphosphate (ALDOB) (AA 1-317) antibody (ABIN393315) Detection limit for recombinant GST tagged ALDOB is approximately 0.3 ng/mL as a capt...
Background publications Bouteldja, Timson: "The biochemical basis of hereditary fructose intolerance." in: Journal of inherited metabolic disease, Vol. 33, Issue 2, pp. 105-12, 2010 (PubMed).

Coffee, Yerkes, Ewen, Zee, Tolan: "Increased prevalence of mutant null alleles that cause hereditary fructose intolerance in the American population." in: Journal of inherited metabolic disease, Vol. 33, Issue 1, pp. 33-42, 2010 (PubMed).

Segat, Milanese, Pirulli, Trevisiol, Lupo, Salizzoni, Amoroso, Crovella: "Secreted protein acidic and rich in cysteine (SPARC) gene polymorphism association with hepatocellular carcinoma in Italian patients." in: Journal of gastroenterology and hepatology, Vol. 24, Issue 12, pp. 1840-6, 2009 (PubMed).

Eriksson, Flach, Lindgren, Kvifors, Lange: "Five mucosal transcripts of interest in ulcerative colitis identified by quantitative real-time PCR: a prospective study." in: BMC gastroenterology, Vol. 8, pp. 34, 2008 (PubMed).

Davit-Spraul, Costa, Zater, Habes, Berthelot, Broué, Feillet, Bernard, Labrune, Baussan: "Hereditary fructose intolerance: frequency and spectrum mutations of the aldolase B gene in a large patients cohort from France--identification of eight new mutations." in: Molecular genetics and metabolism, Vol. 94, Issue 4, pp. 443-7, 2008 (PubMed).